Abstract: The paper aims to provide nutritional and safe quality mulberry branch black fungus and rationally utilize a large number of abandoned mulberry branches to improve the quality safety of black fungus products and promote a healthy and diversified development of edible fungus and sericulture. The conventional nutrients, amino acids, mineral content and heavy metal content of mulberry branch black fungus, wild oak black fungus and ordinary black fungus purchased from market were tested and analyzed with reference to the government standard. The results showed that, the content of crude protein and total sugar of mulberry branch black fungus were higher than that of wild oak black fungus and ordinary black fungus. The crude fat and crude fiber content of mulberry branch black fungus were lower than that of wild oak black fungus and ordinary black fungus. The mulberry branch black fungus contained 17 kinds of amino acids, which included 7 essential amino acids and the total amino acid content was as high as 10.51%. Selenium content in mulberry branch black fungus was higher, which was 0.17 mg/kg. Heavy metals contents, such as lead, mercury, cadmium and arsenic, were very low and met all regulations for food safety. The mulberry branch black fungus has very good nutritional dietary and is se-enriched edible fungi with development prospect. Cultivation of edible fungi by using mulberry branch has positive significance on the recycled development of sericulture, the protection of the ecological environment and the development of edible fungus industry.