Abstract: The study aims to determine the best combination of N application rate, topping mode and remained leaves of flue-cured tobacco variety‘K326’in Qujing, and promote stability of yield and quality and usability in this area. L9(34) orthogonal experimental design was employed to study N application rate, topping mode and remained leaves in Shizong County of Qujing in 2015, and we analyzed the effects of these factors on the agronomic characters, economic characters and chemical composition of tobacco. Results showed that, N application rate was the dominant factor affecting agronomic characters and economic characters. As N application rate increased, the indexes of agronomic characters increased, and N application rate of 120 kg/hm2 was significantly higher than the other two treatments. N application rate of 120 kg/hm2 had the best performance on yield, output value, average price and middle-high quality leaves ratio. Topping mode was the dominant factor affecting smoking quality. Topping at buttoning stage had the maximum yield. Topping at early blooming stage had the best performance on output value, average price, middle-high quality leaves ratio and smoking result. Remained leaves was the dominant factor affecting sensory quality and chemical composition of tobacco, namely, offensive odor and strength of smoke, total sugar, ratios of sugar to alkaloid. N application rate of 120 kg/hm2, topping at early blooming stage and 21 leaves remained was the best combination of this experiment and suitable for practical production in Qujing.