Abstract: To further optimize the industrial cultivation condition of Hupsizygus marmoreus, and screen out excellent strains, 11 Hupsizygus marmoreus cultivars from different places were used as materials. The mycelial growth, the mycelial growth rate and the mycelial growth indexes were compared, and the mycelial growth characteristics of different parent hypha species and original species were studied. The results showed that the mycelial growth of parent hypha species and original species of No.5‘( Hypsizygus marmoreus No.1’) and No.7‘( Hypsizygus marmoreus’) was better than that of others. Their mycelia were dense and uniform and the growth scores were higher, their average growth rate was significantly higher than that of other species. And their mycelial growth indexes were also higher. Therefore, considering the characteristics of mycelial growth, No.5‘( Hypsizygus marmoreus No.1’) and No.7‘( Hypsizygus marmoreus’) could be used as excellent strains for Hypsizygus marmoreus cultivation.