Abstract: The paper aims to understand the distribution of wild Orchidaceae plant resources in Tibet based on the flora of Tibet. Combined with the latest literature and specimen information, the checklist of Orchidaceae in Tibet was combed and sorted out. The results showed that: there were 336 species from 89 genera in Tibet, out of which, 187 species were terrestrial, 136 species were epiphytic and 13 species were mycoheterotrophic; from 2010 to 2017, 15 genera and 19 species of orchids were recorded, of which there were 8 new species and 11 new record species. This study further supplemented the catalogue of Orchidaceae in Tibet. The updated of this checklist will provide a scientific basis for the introduction, domestication and biodiversity conservation of Orchidaceae.