Abstract: In recent years, to achieve sustainable development in the increasingly severe competition, enterprises have gradually moved towards industrial clustering, accompanied by the development of industrial clusters greatly supported by the state and the government. In some areas, enterprises in animal husbandry and food industry cluster have faced a series of problems such as severe homogenization of products, excessive competition and resource plunder. Based on the enterprise ecological niche theory, this paper extracts the first, second and third ecological factors that affect the ecological niches of animal husbandry and food enterprises, and uses AMOS 22.0 analysis software to build a structural equation model (SEM) to perform validation factor analysis. After the model is tested and corrected, 6 secondary ecological factors and 22 tertiary ecological factors affecting the ecological niches of enterprises in animal husbandry and food industry cluster are identified.