Determination of Ergosterol in Fungi and Its Products by HPLC

doi:10.11923/j.issn.2095-4050.cjas20191000244

Abstract

Abstract:

The aim is to find out the characteristic indexes of fungi and its products for quality evaluation and adulteration identification. The HPLC method was established for determining ergosterol in fungi and its products. With the established HPLC method, the contents of ergosterol were determined in fungi and its products (Cordyceps sinensis and Ganoderma lucidum spores powder), spirulina and six kinds of plant oil, such as soybean oil. The results showed that there was a good linearity in the range of 5-160 μg/mL of ergosterol, the correlation coefficient was more than 0.999, the detection limit was 0.1 μg/mL, and the average recovery was from 86.32% to 105.24%. Ergosterol was not determined in spirulina and six kinds of plant oil (such as soybean oil), while it was detected in fungi and its products. Ergosterol had specificity in fungi and its products. This study suggests that the method is simple and sensitive, and has good repeatability and stability, thus it can be adopted to quantitatively analyze ergosterol in fungi and its products.

Key words: Fungi and Its Products, Ergostero, Specificity, High Performance Liquid Chromatography (HPLC)

CLC Number:

S567.3

Wang Xiaoqin, Huang Xiuli, Chen Yuejing, Luo Xuan, Hu Jiashan, Weng Jia. Determination of Ergosterol in Fungi and Its Products by HPLC[J]. Journal of Agriculture, 2021, 11(4): 56-61.

Figures/Tables 3

References 30

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试样	麦角甾醇含量/(mg/g)	平均回收率/%	RSD/%
冬虫夏草	3.13	93.25	4.00
蛹虫草	4.99	93.46	2.09
赤灵芝子实体	1.09	94.22	0.88
紫芝子实体	0.46	89.41	4.82
灵芝孢子粉	1.24	93.74	3.60
猪苓	1.21	94.71	1.14
猴头菇	3.13	93.05	2.63
香菇	4.05	94.86	3.12
桦褐	0.27	91.63	3.51
黑木耳	1.82	93.34	2.07
茯苓	0.17	92.29	5.19
灵芝孢子油	2.18	88.88	0.53
螺旋藻	0	101.21	/
大豆油	0	94.17	/
玉米油	0	92.69	/
葵花籽油	0	95.86	/
花生油	0	94.22	/
菜籽油	0	96.67	/
橄榄油	0	92.94	/

试样	麦角甾醇含量/(mg/g)	平均回收率/%	RSD/%
冬虫夏草	3.13	93.25	4.00
蛹虫草	4.99	93.46	2.09
赤灵芝子实体	1.09	94.22	0.88
紫芝子实体	0.46	89.41	4.82
灵芝孢子粉	1.24	93.74	3.60
猪苓	1.21	94.71	1.14
猴头菇	3.13	93.05	2.63
香菇	4.05	94.86	3.12
桦褐	0.27	91.63	3.51
黑木耳	1.82	93.34	2.07
茯苓	0.17	92.29	5.19
灵芝孢子油	2.18	88.88	0.53
螺旋藻	0	101.21	/
大豆油	0	94.17	/
玉米油	0	92.69	/
葵花籽油	0	95.86	/
花生油	0	94.22	/
菜籽油	0	96.67	/
橄榄油	0	92.94	/