Investigation and Detection of the Insect Vector of Sugarcane White Leaf Phytoplasma in Gengma

doi:10.11923/j.issn.2095-4050.cjas2020-0137

Abstract

Abstract:

To investigate the insect vector and dominant population of sugarcane white leaf (SCWL) phytoplasma in Yunnan can enrich the theoretical and technical basis of sugarcane phytoplasma disease control and provide reference for formulating comprehensive control measures for SCWL. The insect vectors of SCWL phytoplasma were investigated using visual search and sweep netting methods and detected by nested PCR in Gengma of Lincang City, Yunnan Province in 2019, where the occurrence of SCWL was the most serious. The investigation and detection results showed that two collected insect vectors Tettigoniella viridis and Clovia conifer were tested positive. They were natural carriers of SCWL phytoplasma, indicating that these two leafhopper species might be the potential insect vectors of SCWL phytoplasma. Meanwhile, the nymph of T. viridis was tested strongly positive and preliminarily identified as the dominant population. In view of the fact that the main transmission ways of SCWL phytoplasma currently are SCWL-infected seedcane and leafhopper insect vector, it is suggested to establish a sugarcane breeding base of disease-free and healthy seedcane and kill leafhopper insect vector in sugarcane planting area in time, so as to control the spread of SCWL from the source and reduce the natural transmission speed in field.

Key words: Gengma, sugarcane white leaf disease, insect vector, phytoplasma, nested PCR

CLC Number:

S432.1

LI Wenfeng, WANG Xiaoyan, CANG Xiaoyan, ZHANG Rongyue, SHAN Hongli, LU Wenjie, WANG Changmi, YIN Jiong, HUANG Yingkun. Investigation and Detection of the Insect Vector of Sugarcane White Leaf Phytoplasma in Gengma[J]. Journal of Agriculture, 2022, 12(5): 6-9.

Figures/Tables 2

References 27

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样品编号	昆虫介体种类及虫态	甘蔗白叶病巢式PCR检测结果
1	条纹平冠沫蝉成虫	–
2	条纹平冠沫蝉成虫	+
3	大青叶蝉若虫	+
4	大青叶蝉成虫	–
5	条纹平冠沫蝉若虫	+
6	条纹平冠沫蝉成虫	–
7	条纹平冠沫蝉成虫	–
8	条纹平冠沫蝉成虫	+
9	大青叶蝉成虫	+
10	大青叶蝉若虫	++
11	大青叶蝉成虫	–
12	条纹平冠沫蝉若虫	–
13	条纹平冠沫蝉成虫	–
14	大青叶蝉成虫	–
PC	阳性对照	++
NC	阴性对照	–
CK	空白对照	–

样品编号	昆虫介体种类及虫态	甘蔗白叶病巢式PCR检测结果
1	条纹平冠沫蝉成虫	–
2	条纹平冠沫蝉成虫	+
3	大青叶蝉若虫	+
4	大青叶蝉成虫	–
5	条纹平冠沫蝉若虫	+
6	条纹平冠沫蝉成虫	–
7	条纹平冠沫蝉成虫	–
8	条纹平冠沫蝉成虫	+
9	大青叶蝉成虫	+
10	大青叶蝉若虫	++
11	大青叶蝉成虫	–
12	条纹平冠沫蝉若虫	–
13	条纹平冠沫蝉成虫	–
14	大青叶蝉成虫	–
PC	阳性对照	++
NC	阴性对照	–
CK	空白对照	–