Screening of Carbon Dioxide Injury Characteristic Components in Fresh-cut Broccoli

doi:10.11923/j.issn.2095-4050.cjas2024-0103

Abstract

Abstract:

This study aims to identify the characteristic components of carbon dioxide injury in fresh-cut broccoli and establish a foundation for early warning and precise control of CO₂ concentration, thereby enhancing the effectiveness of air conditioning and preservation. Gas chromatography-mass spectrometry was employed to analyze the trend of volatile components produced by broccoli under dynamic controlled atmosphere storage. The results indicated that fresh-cut broccoli sustained injury when stored at 0°C with 4% O₂ and 3% or higher CO₂ concentration, with the injury phenotype being off-odor. The contents of propionic acid specifically increased under injured conditions, displaying a significantly different trend compared to hypoxic conditions. Thus, propionic acid can be used as a characteristic component of carbon dioxide injury in fresh-cut broccoli.

Key words: fresh-cut, broccoli, carbon dioxide injury, characteristic components, propionic acid

ZHU Chunrong, WANG Qingguo. Screening of Carbon Dioxide Injury Characteristic Components in Fresh-cut Broccoli[J]. Journal of Agriculture, 2025, 15(8): 77-87.

Figures/Tables 11

References 31

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序号	物质名称	保留时间/min	序号	物质名称	保留时间/min
1	乙醇	3.83	11	二甘醇	28.625
2	甲苯	6.612	12	四乙二醇二乙醚	29.284
3	二甲基二硫醚	7.044	13	二甘醇单乙醚	29.291
4	十二烷	7.439	14	3,6,9,12-四氧十四烷-1-醇	29.293
5	对二甲苯	8.542	15	12-冠醚-4	30.735
6	右旋柠檬烯	10.181	16	四乙二醇	33.31
7	苯乙烯	11.995	17	正十六烷酸	34.782
8	丙酸	20.194	18	十八烷酸	36.954
9	己酸己酯	21.46	19	五乙二醇	38.766
10	α-法呢烯	24.666	-	-	-

序号	物质名称	保留时间/min	序号	物质名称	保留时间/min
1	乙醇	3.83	11	二甘醇	28.625
2	甲苯	6.612	12	四乙二醇二乙醚	29.284
3	二甲基二硫醚	7.044	13	二甘醇单乙醚	29.291
4	十二烷	7.439	14	3,6,9,12-四氧十四烷-1-醇	29.293
5	对二甲苯	8.542	15	12-冠醚-4	30.735
6	右旋柠檬烯	10.181	16	四乙二醇	33.31
7	苯乙烯	11.995	17	正十六烷酸	34.782
8	丙酸	20.194	18	十八烷酸	36.954
9	己酸己酯	21.46	19	五乙二醇	38.766
10	α-法呢烯	24.666	-	-	-

保留时间/min	相对含量/%					物质名称
保留时间/min	1% CO₂	2% CO₂	3% CO₂	4% CO₂	5% CO₂	物质名称
3.83	1.57	1.23	3.10	2.61	2.55	乙醇
6.612	0.58	0.72	1.12	0.81	0.79	甲苯
7.044	0.66	0.66	0.88	1.16	1.36	二甲基二硫醚
7.439	0.66	0.63	0.77	0.67	0.84	十二烷
8.542	2.47	2.60	3.01	2.40	2.25	对二甲苯
10.181	0.64	0.44	0.68	0.88	0.49	右旋柠檬烯
11.995	0.61	0.56	0.91	0.47	0.40	苯乙烯
20.194	0	0.32	0.81	0.86	0.99	丙酸
21.46	0.54	0.42	0.68	0.69	0.37	己酸己酯
24.666	0.52	0.60	0.46	0.91	0.56	α-法呢烯
28.625	1.34	1.16	2.28	1.59	1.94	二甘醇
29.284	19.20	12.99	16.42	9.32	5.56	四乙二醇二乙醚
29.291	0.98	0.95	1.19	2.17	1.55	二甘醇单乙醚
29.293	2.97	4.16	2.39	3.15	1.39	3,6,9,12-四氧杂十四烷-1-醇
30.735	0.54	0.99	3.11	1.69	0.71	12-冠醚-4
33.31	2.38	2.43	2.81	3.12	2.80	四乙二醇
34.782	34.44	16.10	16.04	20.55	38.62	正十六烷酸
36.954	12.02	14.16	12.76	15.61	38.50	十八烷酸
38.766	33.88	13.61	35.41	18.75	15.31	五乙二醇

保留时间/min	相对含量/%					物质名称
保留时间/min	1% CO₂	2% CO₂	3% CO₂	4% CO₂	5% CO₂	物质名称
3.83	1.57	1.23	3.10	2.61	2.55	乙醇
6.612	0.58	0.72	1.12	0.81	0.79	甲苯
7.044	0.66	0.66	0.88	1.16	1.36	二甲基二硫醚
7.439	0.66	0.63	0.77	0.67	0.84	十二烷
8.542	2.47	2.60	3.01	2.40	2.25	对二甲苯
10.181	0.64	0.44	0.68	0.88	0.49	右旋柠檬烯
11.995	0.61	0.56	0.91	0.47	0.40	苯乙烯
20.194	0	0.32	0.81	0.86	0.99	丙酸
21.46	0.54	0.42	0.68	0.69	0.37	己酸己酯
24.666	0.52	0.60	0.46	0.91	0.56	α-法呢烯
28.625	1.34	1.16	2.28	1.59	1.94	二甘醇
29.284	19.20	12.99	16.42	9.32	5.56	四乙二醇二乙醚
29.291	0.98	0.95	1.19	2.17	1.55	二甘醇单乙醚
29.293	2.97	4.16	2.39	3.15	1.39	3,6,9,12-四氧杂十四烷-1-醇
30.735	0.54	0.99	3.11	1.69	0.71	12-冠醚-4
33.31	2.38	2.43	2.81	3.12	2.80	四乙二醇
34.782	34.44	16.10	16.04	20.55	38.62	正十六烷酸
36.954	12.02	14.16	12.76	15.61	38.50	十八烷酸
38.766	33.88	13.61	35.41	18.75	15.31	五乙二醇