Abstract: Abstract: This study aimed to development the inocula of straw bio-reactor technology in Tianjin. The research focued on the comparison of two kinds of strain application in bio-reactor technology of straw. The result showed that the application effect of two strain in the straw bio-reactor technology was good. Two strain both could improve the greenhouse soil temperature, the photosynthesis and yield of vegetables. The application effect of Tianjin strain in the tomato straw bio-reactor technology planting test was better than Shandong strain, the percentage of increasing yield was 2.80%.And the application effect of Shandong strain in the watermelon straw bio-reactor technology planting test was better than Tianjin strains, the percentage of increasing yield was 4.93%.