Abstract:

In order to study the inhibitory effect of extracts from walnut peel on 4 kinds of plant fusarium wilt pathogens, petroleumether, chlorform and methanol were used to extract the walnut peel, the antimicrobial activity of different extracts were tested with Fusarium oxyspoumf .sp. melongenae, Fusarium oxysporiumf. sp. cucumeris, Fusarium oxysporumf. sp. pepper and Fusarium oxysporiumf. sp watermelon, respectively. The results showed that the methanol extracts of walnut peel had the strongest inhibitory effect on tested fusarium wilt, the inhibition rates were more than 60% to Fusarium oxyspoumf. sp. melongenae, Fusarium oxysporiumf. sp. cucumeris and Fusarium oxysporumf. sp. pepper, was 37.9% to Fusarium oxysporiumf. sp watermelon. The petroleum ether extracts from methanol extract of walnut peel had stronger antimicrobial activity with EC50 of 4.45 mg/mL against Fusarium oxysporiumf. sp watermelon, the inhibition rate was 65%, the inhibition rates of the chlorform extracts against Fusarium oxyspoumf. sp. melongenae and Fusarium oxysporiumf. sp .cucumeris were 65% and 62.3%，EC50 were 4.46 mg/mL and 3.51 mg/mL; the inhibition rate of the methanol extracts against Fusarium oxysporumf. sp. pepper was 63.94%, the EC50 values of methanol’s extraction against mycelial growth of Fusarium oxysporumf. sp. pepper was 4.23 mg/mL.