Abstract:

The optimal conditions for the ultrasonic extraction of trigonelline from Trigonella coerulea was studied in the present study. Taking the yield of trigonelline as index, the content of trigonelline was determined by HPLC. On the basis of single factor experiments, the four major effect factor, including extraction temperature, the extraction time, the ultrasonic power and the methanol concentration were selected to optimize the extraction conditions using uniform design. The results were analyzed through SPSS software. The optimal conditions for trigonelline extraction were explored to be ultrasonically extracted by 48% methanol at 64℃ and 120 W ultrasonic power for 75 min. The ultrasonic extraction is efficient, rapid for trigonelline with high yield.