Abstract:

In the present study, Alcalase protease and Protex.7L protease were utilized to hydrolyze soybean protein in different ways. i.e., single-enzyme hydrolysis as well as one-step and stepwise double-enzyme hydrolysis. After degree of hydrolysis, protein utilization, peptides yield, oligopeptides yield were measured to comparatively study soybean protein hydrolysate in different hydrolysis way. The results showed that stepwise double-enzyme hydrolysis was superior to single-enzyme hydrolysis and one-step double-enzyme hydrolysis, and sequential hydrolysis with Alcalase protease followed by Protex.7L protease was the best way. Under this way, the degree of hydrolysis, protein utilization, peptides yield, oligopeptides yield reached 23.5%, 68.7%, 51.0%, 21.4%.