Abstract: Distribution of whiteflies in single-span greenhouses were investigated in order to prvideprovide fundamental information for the control of whiteflies, this study investigated the distribution of whiteflies in single-span greenhousese. Our results indicated that the damage of adults, eggs and low instar nymphs of whiteflies were concentrated in the middle and upper part of the plant while large number of pupas were accumulated on the bottom part of the plant. The damage of adults, eggs and low instar nymphs of whiteflies in the middle and upper part of the plant in the middle ridge were more serious than that of the edge ridge. All in all, remove old leaves, chemical control focused on the middle and upper part of the plant, development of integrated management strategies of physical and biological methods together with the whitefly control in the middle ridge are effective ways for the prevention of whitefly in single-span greenhouses.