Abstract: The target of tobacco cultivation is excellent quality and optimal yield. Balanceable development of carbon and nitrogen metabolism is necessary condition of excellent quality of tobacco leaves. Nitrogen is important element to regulate carbon and nitrogen metabolism, yield and quality. We could regulate nitrogen account to control carbon and nitrogen metabolism of tobacco leaf, and then to achieve the target of excellent quality and optimal yield. This paper reviews the relation of carbon and nitrogen metabolism and nitrogen status in ripening and curing period of tobacco leaf to tobacco leaf quality, and provide suggestion to regulate carbon and nitrogen metabolism and then improve the tobacco leaf quality by control nitrogen fertilization.