Abstract: The database was constructed based on the Flora of cotton varieties in China (1978-2007) and official variety registration announcement.. It collected 839 cotton varieties (Gossypium hirsutum) with 14 agronomic trait such as growth period, plant height, sympodial branch nod, boll weight, lint percentage, lint yield and fiber quality, which authorized and promoted in Yangtze River, Yellow River and Northwestern inland cotton production zone, The statistical analysis demonstrated that the difference of fiber length, lint percentage, growth period, micronaire and boll weight was not significant among varieties in three major cotton production zones; But the difference of fusarium wilt resistance, verticillium wilt resistance, leaf color and planting density was significant. Varieties with highest fusarium wilt resistance and verticillium wilt resistance of varieties were from Northwestern production zone, and the lowest were from Yangtze River production zone. The planting density was highest in Northwestern zone, followed by Yellow River zone and Yangtze River zone; the fiber strength was highest in Northwestern zone, followed by Yangtze River zone and Yellow River zone, and the different varieties presented significant difference in the same production zone. The fiber yield showed the same law as fiber strength. Therefore, this study suggested that varieties with good fiber quality were more suitable developed in northwestern cotton production zone, especially in Xinjiang province, and more disease resistant varieties should be developed in Yangtze River cotton production zone.