Abstract: SRAP was used to analyse the genetic diversity analysis of 42 Cymbidium goeringii cultivars and 1 specie of Cymbidium floribundum、1 specie of Cymbidum.longibracteatum and 3 species of Hybrid cymbidium. 15 polymorphic primer combinations were screened and a total of 185 DNA loci were amplified，184 of which were polymorphic. The average number of polymorphic DNA loci amplified by each primer was 12.27. Primer combination Me8-Em16’s Nei’s genetic diversity index(H,0.3993) ,Shannon’s information index( I, 0.5794) and Effective number of alleles(Ne, 1.7280) were the highest. The genetic similarity among 47 germplasm ranged from 0.63~0.80. The UPGMA clustering showed that all the tested cultivars and species were classified into five cluster groups with the similarity coeficient of 0.662.The study were well revealed the 47 materials genetic relatives..The findings of this study would provide an important theoretical basis for Cymbidium goeringii classification and parental selection in cross breeding．