Abstract: The total nitrogen content of flue-cured tobacco from main tobacco-growing areas in Qianxinan was determined by continuous flow method to study its space-time characteristic. The results showed that total nitrogen content of flue-cured tobacco belonged to lower-sugar tobacco-planting areas with a mean of 1.78%, amplitude of 1.36%-2.42%. The total nitrogen content of flue-cured tobacco in Zhenfeng County, Qinglong County, Xingyi City was extremely significant more than Xingren County. The total nitrogen content increased with the increase of the year. The total nitrogen content decreased gradually from the high total nitrogen content areas northeast of to southwest orientation.