Abstract: A soil culture experiment was carried out to study the effects of different biochar application rates of 0.75%, 1.5% and 2.0% on the content of effective antibiotics in soil, the uptake amount of antibiotics by vegetables and the growth of vegetable. The soil was treated with 4 antibiotics (oxytetracycline, enrofloxacin, sulfamethazine, and tylosin) with the concentration of 25 mg/kg. The results showed that the application of biochar could slow down the degradation of antibiotics, but it significantly decreased the concentration of available antibiotics in soil. After 52 days incubation, the application of 1.5% and 2.0% biochar decreased the concentrations of soil available antibiotics by 25.29% and 39.09% for oxytetracycline, 16.39% and 28.69% for enrofloxacin, 16.97% and 23.64% for sulfamethazine and 17.76% and 25.70% for tylosin, respectively. The application of biochar could also reduce the uptake amount of antibiotics by vegetable plants. After the application of 1.5% and 2.0% biochar, antibiotics content in vegetable reduced by 23.11% and 35.93% for oxytetracycline, 15.89% and 22.85% for enrofloxacin, 7.64% and 16.56% for sulfamethazine and 16.79% and 29.50% for tylosin, respectively. The application of biochar could increase the biomass of vegetable plants compared to CK.