Sensitivity of Gibberella zeae(Schwein.)Petch to carbendazim and correlation to fungicides

doi:10.11923/j.issn.2095-4050.cjas16040033

Abstract

Abstract: In order to investigate the resistance of Gibberella zeae (Schwein.) Petch to carbendazim and the cross resistance to carbendazim and other fungicides, the drug resistance of 520 isolates of G.zeae (Schwein.) Petch collected from 26 counties (cities) in Jiangsu Province were detected by the method of distinguishing dosage method, and then 10 isolates with different sensitivities to carbendazim were used to study the cross resistance to azoxystrobin, pyraclostrobin, trifloxystrobin, pyrametostrobin, epoxiconazole, hexaconazole, triticonazol and fludioxonil by the method of mycelium growth rate method. The results showed that the resistance frequency of G.zeae (Schwein.) Petch of different counties (cities) in Jiangsu was different, and the total resistance frequency was 50.58%; correlation analysis of EC50 showed that there was no cross resistance between carbendazim and other fungicides. These results suggested that G.zeae (Schwein.) Petch in Jiangsu had relatively high resistance frequency to carbendazim, and it was urgent to screen new fungicide to control fusarium head blight.

References

[1]Maier F J, Malz S, Losch A P, et al. Development of a highly efficient gene targeting system for Fusarium graminearum using the disruption of a polyketide synthase gene as a visible marker[J]. FEMS Yeast Res,2005,5:653–662.
[2]Proctor R H, Hohn T M, McCormick S P. Restoration of wildtype virulence to Tri5 disruption mutants ofGibberella zeae via gene reversion and mutant complementation[J]. Microbiology,1997, 143:2583-2591.
[3]Atanassov Z, Nakamura C, Mori N, et al. Mycotoxin production and pathogenicity of Fusarium species andwheat resistance to Fusarium head blight[J]. Can J Bot ,1994,72:161-167.
[4]Chen C J,Wang J X, Luo Q Q,et al. Characterizationand fitness of carbendazim-resistant strains of Fusarium graminearum (wheat scab)[J]. Pest Manag Sci,2007, 63:1201–1207 .
[5]Liu X, Yin Y N, Wu J B,et al.Identification and characterization of carbendazim-resistant isolates of Gibberellazeae[J]. Plant Dis,2010,94:1137–1142.
[6]王建新,周明国,陆悦健,等.小麦赤霉病菌抗药性群体动态及其治理药剂[J]. 南京农业大学学报,2002,25(1):43-47.
[7]Liu Y, Chen X, Jiang J H,et al.Detection and dynamics of different carbendazim-resistance conferring β-tubulinvariants of Gibberella zeae collected from infected wheat heads and rice stubble in China[J]. Pest manag Sci,2014,70:1228-1236.
[8]Qu B, Li H P, Zhang J B, et al. Comparison of genetic diversity and pathogenicity of Fusarium head blight pat-hogens from China and Europe by SSCP and seedling assays on wheat[J]. Plant Pathol,2008,57:642–651.
[9]韩青梅,康振生,黄丽丽,等.戊唑醇对小麦赤霉菌侵染影响的细胞学研究[J].植物保护学报,2005,32(1):57-62.
[10]毕秋艳,马志强,张小风,等.福美双和戊唑醇增效与拮抗组合对小麦赤霉病菌细胞膜透性及内含物渗漏的影响[J].中国农学通报,2009,25(20):222-227.
[11]刁亚梅,倪珏萍,马亚芳,等. 创制杀菌剂氰烯菌酯的应用研究[J].植物保护,2007,33(4):121-123.
[12]侯昌亮,艾爽,姚安庆,等. 5种杀菌剂对小麦赤霉病菌的室内毒力测定[J].长江大学学报(自然科学版),2012,9(12):1-2.
[13]侯昌亮,胡寒哲,艾爽,等.11种杀菌剂对小麦赤霉病菌的抑制作用[J].湖北农业科学,2014,53(17):4066-4068.
[14]赵斌,何绍江. 微生物学实验[M]. 北京:科学出版社,2002:251.
[15]张昊,张争,许景升,等.一种简单快速的赤霉病菌单孢分离方法——平板稀释画线分离法[J].植物保护,2008,34(6):134-136.
[16]石志琦,史建荣,陈怀谷,等. 小麦赤霉病菌对多菌灵的抗药性研究[J]. 农药学学报,2000,2(4):22-27.
[17]王建新,周明国. 小麦赤霉病菌对多菌灵抗药性监测技术研究[J]. 植物保护学报,2002,29(1):73-77.
[18] Xiao K H,Gregory E, Sany R, et al.Effect Of famoxadone on photoinduced electron transfer between the Iron-Sulfur center and cytochrome c1 in the cytochrome bcl complex[J].The Journal of Biological Chemistry,2003,278:11419-11426.
[19]Lothar E, Byron Q, Li Y F,et al. Crystallographic studies of quinol oxidation site inhibitors: a modified c1assi-ficadon of inhibitors for the cytochrome bcl complex[J]. Journal of Molecular Biology,2004,341:281-302.
[20]翟平平,刘友超,姚安庆.小麦赤霉病杀菌剂室内筛选试验[J].长江大学学报(自然科学版),2012,9(3):1-2,14.
[21]金丽华,陈长军,王建新,等.嘧菌酯及SHAM对4种植物病原真菌的活性和作用方式研究[J]. 中国农业科学,2007,40(10):2206-2213.
[22]杨瑞秀,司乃国. 唑胺菌酯(SYP-4155)作用特性研究.//周明国,陈长军.中国植物病害化学防治研究(第6卷)[M].北京:中国农业科学技术出版社,2008:25-28.
[23]刘颖超,张金林,庞民好,等. 咯菌腈对草莓灰霉病Botrytis cinerea的毒力及防效研究初报[J]. 农药学学报,2002,4 (3):94-96.