Abstract: The study aims at providing references for the rapid propagation system establishment of tissue culture of Garcinia multiflora Champ. By means of observing the comparative experiments, completely randomized orthogonal experiment design, analysis of variance (ANOVA), significance test and LSD multiply comparative analysis, the control of the most optimal sterilization time, the optimal primary culture medium type and hormone concentration formula were discussed. The results showed that when the sterilization time of explants was 2 min, the pollution rate was the highest, while the mortality rate and survival rate were relatively low. When the sterilization time was 6 min, the pollution rate and mortality rate were medium, while the survival rate was the highest. When the time was 10 min, the pollution rate and survival rate were the lowest, while the mortality rate was the highest. MS and B5 had better effect on the primary culture medium of Garcinia multiflora Champ. If MS and B5 were respectively added with different concentrations of 6-BA and NAA, the induction effect of adventitious buds of explants was different. The maximum proportion of MS was 6-BA 1.6 mg/L + NAA 0.9 mg/L, and the best ratio of B5 was 6-BA 1.6 mg/L + NAA 0.6 mg/L. Taken together, the optimum sterilization time of explants is 6 min, MS + 6-BA 1.6 mg/L + NAA 0.9 mg/L has the best culture effect.