Abstract: In this paper, the feasibility of single seed sowing was verified in floating seedling system. A field experiment was conducted with primed and pelleted seeds of flue-cured tobacco as the test materials. Three treatments were set up: a manual integrated sowing device which had planting hole-making, sowing, and seed covering (T1), a manual sowing device with separated planting hole-making, sowing, and seed covering (T2), and machine sowing (CK). At the time of sowing, one seed was sown in one hole by manual sowing device, while two seeds were sown in the surrounding hole and one seed was sown in the remaining hole by machine sowing. The experimental data of germination rate, double seedlings rate, multiple seedlings rate, the labor amount and the labor cost during the period of the 4 true leaves were estimated and analyzed using random sampling. The results showed that the germination rate, double seedling rate and multiple seedlings rate of machine sowing was 85.9%, 32.4% and 13.4%, respectively.Thecostofthinningseedlingsandfillingseedlingswas 0.8yuan/plate in machine sowing. The germination percentage was more than 90% by manual sowing device, the minimum germination percentage was 91.7% in January, and the cost of thinning seedlings and filling seedlings could be saved. Therefore, the manual sowing device, which set planting hole-making, sowing, and seed covering in integration, was the best, not only to enhance the germination percentage, uniformity and quality of tobacco seedling, but also to reduce the production cost and the risk of virus spread, and save seeds.