Abstract: Distiller grains contain lots of nutrients, which are capable for efficient livestock feed production. But the crude fat content of distiller grains is too high, so the grains are not suitable for producing high protein feed. For rational utilization of distiller grains, the authors used three strains (27435 strain, our separation strain and 30942 strain) to treat distiller grains, and screened out the best one for reducing vinasse crude fat content and increasing the protein content. The results sho ed that the 24735 strain reduced crude fat content effectively, and had certain effect on the nutrition of vinasse.