Abstract: With Hu sheep as the research object, we designed three different doses of ApoB antigens. The antibody production, antibody titer and ApoB product performance were analyzed. The results showed that the antibody titer of group 2 (0.4 mg antigen each time) was significantly higher than those of group 1 (0.2 mg antigen each time) and that of group 3 (1 mg antigen each time). Antibody titer could reach 1:29 after five times of immunization in group 2. The indexes of ApoB production performance were qualified, which indicated that the best efficiency of ApoB antibody was produced when each immunization antigen was 0.4 mg ApoB antigen. This study laid a good foundation for the wide utilization of ApoB antibody in medical field.