Abstract: The paper aims at the edible fungi production problems of fruiting time concentration and nutrient remaining after harvesting. The authors took oyster and phoenix mushroom as experimental materials, and used natural storage and second intermittent fruiting to study the regulation and controlling of harvest times. The results showed that, the biological efficiency of normal fruiting for oyster mushroom cultivation ranged from 41.923% to 129.1% and there was a difference of more than three times; the biological efficiency of normal fruiting for phoenix mushroom cultivation ranged from 64.31% to 78.77%, and there was little difference. From the aspect of experimental result of fruiting time controlling, the biological efficiency of oyster mushroom variety‘831’was reduced from 50.312% for normal fruiting to 46.591% for delayed controlling fruiting, the biological efficiency directly reduced by 3.721 percentage points and 7.4%; the biological efficiency of phoenix mushroom variety‘195’was also reduced from 69.4% for normal fruiting to 28.99% for delayed controlling fruiting, the biological efficiency was reduced more than half. The intermittent secondary fruiting experiment with oyster mushroom variety of‘803’showed that the two treatments of‘thin box planting’and‘direct wholly- skinned’had the highest yield of mushroom fruit body, the biological efficiency was 12.42% and 16.08% respectively, and the biological efficiency for other fruiting methods was only about 1%. Therefore, oyster and phoenix mushroom is suitable edible fungi species for the fruit body fruiting time controlling, and have important values in mushroom production, but the intermittent secondary fruiting technique has little production significance due to the large amount of labor used and low yield.