Abstract: Perforation disease has become one of the important leaf diseases of Amygdalus mira that severely affects its growth and life span in Linzhi, Tibet. In 2017 and 2018, the A. mira perforation disease incidences were investigated in different regions of Linzhi, to clarify the occurrence and pathogenic factors of the disease. The results showed that A. mira perforation disease was commonly occurred in all investigated areas, and different regions had different degrees of disease incidence. The average leaf rate and disease index were 26.85% and 9.28, respectively. As also, the disease was initially and severely occurred at the end of June and August, respectively, and the highest leaf rate was 89.45% at the end of August. In addition, the altitude, relative humidity, tree age and living habitat were considered as the main factors that related to the disease occurrence. Under the condition of lower altitude, higher relative humidity, more closed ecological environments and older trees, the disease could be more serious. The study can provide references for controlling A. mira perforation disease in Linzhi, Tibet.