Abstract: To clarify the effects of fertilization on the accumulation of N, P and K in‘Yulu Xiang’pear during different growing stages, 10- year- old‘Yulu Xiang’pear tree was selected as the research object, and experimental groups with different fertilizer ratios of N, P and K were set up. The results found that N, P, and K level showed a downward trend with the growth and development of‘Yulu Xiang’pear trees. N fertilizer increased the level of N, P and K in roots in the germination stage and in roots, leaves in the fruit-expanding stage, and inhibited the accumulation of N and P in roots during the flowering stage. K fertilizer could promote the accumulation of K in roots of in the germination and fruit-setting stage, but down-regulated N and P level in roots in the germination stage, K level in roots in the flowering stage. P fertilizer significantly inhibited the accumulation of P and N in roots in the germination stage, and promoted P and N level in the flowering stage, and N, P and K level in the fruit enlargement stage. The present study suggests that the accumulation of N, P and K in the roots and leaves are affected by the fertilizer composition and growth cycle of‘Yulu Xiang’ pear, and interaction effect of different factors should be fully considered in fertilization.