Mapping Cold-Tolerant QTL in Rice at Seedling Stage: Using a High-Density Genetic Map of Recombinant Inbred Lines

doi:10.11923/j.issn.2095-4050.cjas2020-0079

Abstract

Abstract:

To map the cold-tolerant QTL in rice, the recombinant inbred lines (RILs) population derived from the cross between ‘Wufeng B’ and ‘Changhui T025’, the parents of double-cropping super rice ‘Wufengyou T025’, were used as the materials. The survival rate, root number, root length and bud length at young seedling stage treated at low temperature of 10℃ were determined. Three QTL, qRL1, qRL2, and qBL6 controlling the cold tolerance of rice at seedling stage, were detected by QTL Icimapping v4.2 software. These QTL were identified on chromosome 1, 2 and 6, respectively, their LOD values were 2.98, 2.51 and 5.26, respectively, and the phenotypic variation explained by these QTL were 10.54%, 8.67% and 14.04%, respectively, and the positive alleles of the three QTL were all from ‘Changhui T025’. These QTL were mapped at an interval of 6.75k-40.05kb on Nipponbare genome, which laid the foundation for the improvement of indica cold tolerance at young seedling stage by using these favorable QTL via marker-assisted selection. Furthermore, 13 pairs of epistatic interaction QTL were detected on all 12 chromosomes, and the epistatic interaction between chromosome 3 and chromosome 8 accounted for 21.77% of the phenotypic variation. The results indicate that the epistatic interaction effect plays an important role in rice cold tolerance at young seedling stage.

Key words: Rice, Recombinant Inbred Lines (RILs), Seedling Stage, Cold Tolerance, QTL Analysis

CLC Number:

S330

Liu Ruiqi, Zou Andong, Wu Guangliang, Wang Peng, Li Caijing, Zhang Xiangyu, Cheng Qin, Wang Yanning, Huang Shiying, He Haohua, Bian Jianmin. Mapping Cold-Tolerant QTL in Rice at Seedling Stage: Using a High-Density Genetic Map of Recombinant Inbred Lines[J]. Journal of Agriculture, 2020, 10(7): 7-14.

Figures/Tables 9

References 32

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染色体	标记数目	标记间平均距离/Mb	标记间最小距离/Mb	标记间最大距离/Mb
chr01	303	0.61	0.03	3.43
chr02	285	0.69	0.04	4.30
chr03	235	0.76	0.05	3.40
chr04	240	0.67	0.02	6.51
chr05	177	0.86	0.07	5.21
chr06	226	0.59	0.02	3.89
chr07	180	0.70	0.01	4.33
chr08	159	0.92	0.07	5.28
chr09	171	0.72	0.05	5.28
chr10	166	0.64	0.06	3.26
chr11	208	0.60	0.04	3.74
chr12	228	0.58	0.02	7.24
Average	215	0.70	0.04	4.66

染色体	标记数目	标记间平均距离/Mb	标记间最小距离/Mb	标记间最大距离/Mb
chr01	303	0.61	0.03	3.43
chr02	285	0.69	0.04	4.30
chr03	235	0.76	0.05	3.40
chr04	240	0.67	0.02	6.51
chr05	177	0.86	0.07	5.21
chr06	226	0.59	0.02	3.89
chr07	180	0.70	0.01	4.33
chr08	159	0.92	0.07	5.28
chr09	171	0.72	0.05	5.28
chr10	166	0.64	0.06	3.26
chr11	208	0.60	0.04	3.74
chr12	228	0.58	0.02	7.24
Average	215	0.70	0.04	4.66

性状	亲本		RILs群体
性状	昌恢T025	五丰B	均值	标准误	偏度	峰度	最小值	最大值
根长	3.94	6.04**	6.41	1.19	0.09	0.25	3.61	9.97
芽长	4.05	4.93^ns	5.21	0.64	0.27	0.02	3.7	7.08
根数	5.5	6.9**	6.35	0.78	-0.07	0.41	4.1	8.44
存活率	100%	100%	90.34%	0.2	-3.84	15.06	0	100%

性状	亲本		RILs群体
性状	昌恢T025	五丰B	均值	标准误	偏度	峰度	最小值	最大值
根长	3.94	6.04**	6.41	1.19	0.09	0.25	3.61	9.97
芽长	4.05	4.93^ns	5.21	0.64	0.27	0.02	3.7	7.08
根数	5.5	6.9**	6.35	0.78	-0.07	0.41	4.1	8.44
存活率	100%	100%	90.34%	0.2	-3.84	15.06	0	100%

性状	根长	芽长	根数	存活率
根长	1	0.39^**	0.07	0.14
芽长		1	0.23^**	0.10
根数			1	0.04
存活率				1