An Autotoxicity p-Hydroxybenzoic Acid-degrading Strain ZH2: Isolation and Application

doi:10.11923/j.issn.2095-4050.cjas2020-0115

Abstract

Abstract:

Autotoxicity is the main factor causing obstacles to continuous cropping plants. The aim of the study is to screen bacteria from soil which can degrade autotoxicity. A selective screening method was used to screen p-Hydroxybenzoic acid (PHBA) degradation bacterium. Bacterial species were identified by combining the morphological characteristics, physiological and biochemical characteristics and 16S rRNA sequencing results. The ability of the strain to degrade PHBA was determined by ultraviolet spectrophotometry. The effect of detoxification was verified by a pot experiment of cucumber. The results showed that one degradation strain (No. ZH2) was isolated and identified as Pseudomonas chlororaphis. On the condition of pure culture, ZH2 could degrade 97% at 72 h under 5 mg/mL PHBA concentration. In potted conditions, when the concentration of PHBA in the substrate was 10 mg/g, ZH2 could effectively alleviate the growth inhibition of PHBA on cucumber. We report a Pseudomonas chlororaphis strain screened from soil can degrade PHBA, it has the potential value of controlling continuous cropping obstacles.

Key words: p-Hydroxybenzoic Acid, Autotoxicity, Pseudomonas chlororaphis, Continuous Cropping Obstacle, Degradation

CLC Number:

Xiao Rong, Deng Shu, Zhao Jing, Zhang Chunfen, Nie Yuanjun, Cao Qiufen. An Autotoxicity p-Hydroxybenzoic Acid-degrading Strain ZH2: Isolation and Application[J]. Journal of Agriculture, 2021, 11(7): 84-91.

Figures/Tables 7

References 40

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处理	对羟基苯甲酸含量/(mg/g soil)	ZH2菌液/mL
CK	0	0
PHBA/ZH2	10	20
PHBA	10	0

处理	对羟基苯甲酸含量/(mg/g soil)	ZH2菌液/mL
CK	0	0
PHBA/ZH2	10	20
PHBA	10	0

项目	结果	项目	结果	项目	结果
革兰氏染色	—	卵磷脂酶	+	从蔗糖产生果聚糖	+
氧化酶	+	水解淀粉	—	柠檬酸利用	+
过氧化氢酶	+	明胶液化	+	L-阿拉伯糖利用	+
精氨酸双水解酶	+	非荧光色素	+	乙醇利用	—

项目	结果	项目	结果	项目	结果
革兰氏染色	—	卵磷脂酶	+	从蔗糖产生果聚糖	+
氧化酶	+	水解淀粉	—	柠檬酸利用	+
过氧化氢酶	+	明胶液化	+	L-阿拉伯糖利用	+
精氨酸双水解酶	+	非荧光色素	+	乙醇利用	—

同源性最高菌株				相似度	数据库
名称	登录号	分类名	是否标准菌株	相似度	数据库
ST-TJ4	MT110678.1	P. chlororaphis subsp. aurantiaca	否	100%	NCBI
Sh2	JX477174.1	P. chlororaphis subsp. aureofaciens	否	100%	NCBI
DSM19603	CP027746	P. chlororaphis subsp. aurantiaca	是	100%	EZbiocloud
NBRC3521	BBQB01000031	P. chlororaphis subsp. aureofaciens	是	99.91%	EZbiocloud
NBRC3904	BCZX01000031	P. chlororaphis subsp. chlororaphis	是	99.82%	EZbiocloud