Abstract:

The objective is to breed new varieties of flue-cured tobacco, improve the yield and quality of tobacco leaves and meet the needs of tobacco production. A new flue-cured tobacco strain ‘Shiliuyan’ was selected from the local tobacco varieties planted in Shiqian County of Guizhou Province. Using the main commercial varieties ‘K326’ and ‘Yunyan87’ as control, the botanical characters, agronomic traits, economic characters, appearance quality, physical indexes, chemical components and sensory evaluation quality of tobacco leaves of ‘Shiliuyan’ were evaluated through field tests and laboratory quality tests of three consecutive years. The SSR molecular markers of ‘Shiliuyan’ were also established. The results showed that the number of effective leaves per plant of ‘Shiliuyan’ was 24, 3 leaves more than ‘K326’ and 5 leaves more than ‘Yunyan87’, respectively. The economic indicators of ‘Shiliuyan’, including the yield of 2233.4 kg/hm², average price of 24.47 Yuan/kg, production value of 54434.2 Yuan/hm², the rate of superior grade leaves of 51.11%, the rate of superior and middle grade leaves of 94.81%, were better than those of ‘K326’ and ‘Yunyan87’. The color of tobacco leaves of ‘Shiliuyan’ was lemon yellow, and the strain had good appearance quality, good coordination of chemical components and better sensory evaluation quality than those of the control. The SSR molecular marker primer PT3024 and PT40021 could stably amplify specific bands among ‘Shiliuyan’, ‘K326’ and ‘Yunyan87’. In brief, ‘Shiliuyan’ is a new flue-cured tobacco strain with high yield and good quality of tobacco leaves.

Key words: Flue-cured Tobacco (Nicotiana tabacum L.), Selection and Breeding, ‘Shiliuyan’, SSR Molecular Marker