Abstract:

With further study on genetic character of Sarcandra glabra (Thunb.) Nakai, ISSR markers were used to analyze genetic diversity in 37 kinds of Sarcandra glabra (Thunb.) Nakai. Among the 100 ISSR molecular markers, there were 17 primers that showed good diversity and clear bands. The genetic diversity analysis and cluster analysis were based on the amplification results with POPGENE 1.32 and NTSYS software. The results showed that: 105 sites were found by 17 primers, and 87 sites showed polymorphism, the percentage of polymorphic loci was 65.71%., the genetic diversity of the tested five populations: Fujian, Guangdong, Jiangxi, Zhejiang and Guangxi, was 0.1770, and the intra species genetic diversity was 0.0990. The coefficient of gene differentiation (Gst) was 0.4405, and the gene flow Nm was 0.6350. indicating that 44.05% population variation comes from the interspecific variation, and the others comes from the intraspecific variation. The variance range of genetic similarity coefficient were 0.7048 -0.9143, with an average of 0.8096. Cluster analysis showed that 37 kinds of species could be clustered into 5 groups at a genetic distance coefficient of 0.81. The cluster result indicated that the genetic distance between each small population was related to the environment in which different population of Sarcandra glabra (Thunb.) Nakai grew. However,the genetic distance had no relativity with geographic distance.