Abstract: Ran protein is a member of small GTP-binding protein family controlling the transport of protein and RNA, some of them are found to be responsive to different environmental stresses. In order to obtain Ran gene in sweet potato, according to the spliced EST sequences, a pair of specific primer was designed, and Ran gene from peanut (IbRan) was amplified successfully by RT-PCR. After T-A cloning, sequencing result indicated that the cloned cDNA contained a continuous complete open reading frame (ORF) of 666 bp encoding a polypeptide of 221 amino acids with a calculated molecular mass of 25.15 kDa. Sequence comparison of the IbRan showed very high homology to the Ran subfamily from other species, with over 90% homology.Transcription of IbRan gene was differentially regulated by different environmental stimuli such as cold, PEG, NaCl and the plant hormone ABA using real-time PCR. This gene can be used to regulate stresses resistance of sweet potato by genetic engineering.