Abstract: In order to solve dinotefuran SC insufficient content analysis methods, as well as when using liquid chromatography mobile phase selection, instrument conditions to determine the problems encountered when this article Shimadzu LC-10AT liquid chromatograph based, the use of 2.5 cm×4.6 mm stainless steel, built-C18, 5 μm packing, acetonitrile+ water+ acetic acid+ triethylamine as mobile phase dinotefuran suspending a body of content for testing. The results showed that the use of mobile phase conditions and instrumentation, dinotefuran, the content did not exist an effective body isomer peaks overlap. Peak and impurity peaks best peak shape was good. The research showed that 20% dinotefuran SC analytical methods proposed not only to fill the total content of the neonicotinoid insecticide analytical method blank and determination convenient, accurate, feasible and beneficial to producers for quality control.