Abstract: Isolation and identification of pathogenic bacteria that cause Acipenser schrenckii wound infection will provide scientific basis for prevention and treatment of the disease of Acipenser schrenckii. In this study, bacterial flora of putrid meat and liver from a case of wound-infected Acipenser schrenckii which was secondary to minimally invasive surgery was analyzed, and a dominant strain YSJ01 was isolated, and used for morphological test, antibiotic susceptibility test, animal infection test and identification of VITEK automatic micro-analysis system. The results showed that the strain YSJ01 was gram-negative, and was identified as Aeromonas hydrophila by VITEK automatic micro-analysis. The typical symptoms of wound fester appeared on infected Acipenser schrenckii. Antibiotic susceptibility test indicated that strain YSJ01 was susceptive to nine kinds of drugs such as florfenicol, cotrimoxazole, spectinomycin, rifampin, doxycycline and kanamycin etc. While it showed resistance to penicillin, ampicillin, carbenicillin, vancomycin, novobiocin, streptomycin, nalidixic acid, amoxicillin and lincomycin etc. Therefore, this study provided an experimental basis for prevention of disease secondary to wound infection of Acipenser schrenckii in south China.