Abstract: Brasenia schreberi, precious green vegetable, is one of the riching pillar industries of peasants to vegetable exporting and gain foreign currency in Shizhu, Chongqing, while the leaf spot disease of B. schreberi damage occurred and becoming more and more serious. In this study, we carried out the in-vitro antifungal activities testing and plot experiment for an effective fungicide to control the disease. It was showed with antagonistic activity on PDA plates that the growth of the pathogen was inhibited by tebuconazole, difenoconazole, difenoconazole?propiconazole and pyraclostrobin, the mycelial colony growth inhibiting rate reached 75%. The field control efficacy of pyraclostrobin on the leaf spot disease was best. The average relative control effects were 43.6% and 47.8%, respectively, on 8 days and 16 days after the third fungicides application. All fungicides did not present considerable phytotoxicity symptoms. These results suggest that pyraclostrobin is a candidate fungicide to control the leaf spot disease of B. schreberi.