Abstract: Acer Negundo ‘Auratum’ has been cultivated in the three North Region, Jiangsu and Zhejiang area and South China, thanks to its stronger ability to adapt to the soil and climate environment. In the meantime, Acer Negundo ‘Auratum’ is a ornamental species with a high ornamental value and wide range of application, and suitable for courtyard trees, street trees and specimen trees. In order to speed up seedling propagation of Acer Negundo ‘Auratum’, the tender branch cutting propagation technique of Acer Negundo ‘Auratum’ was studied in this study, by making full use of cutting facilities in the colored-leaf trees domestication base of Henan Academy of Agricultural Science. The tender branch cutting has advantages of quick rooting, high survival rate, and a rich source of branches. But cutting facilities and the choice of the substrate is a key to success of the tender branch cutting. The tender branch cutting of Acer Negundo ‘Auratum’ was put by using full sunshine and auto spay system, and perlite substrate, in order to improve the rooting rate and survival rate. The results showed that rooting rate and survival rate reached both above 95%, and rotten root rate was almost zero. Therefore, it is an effective approach to achieve the scale rapid tender branch cutting propagation of Acer Negundo ‘Auratum’ under full sunlight and water spraying, and using perlite as a culture substrate.