Abstract: In order to study the effect of foliar spray of selenium on rice yield and selenium content in rice, the author adopted random plot experiment and sprayed foliar selenium fertilizer on rice at different growth stages, then determined selenium content in rice under different treatments by atomic fluorescence spectrometry, and calculated rice yield in plot. The results showed that spraying Se fertilizer had no effect on effective panicle number and grain number per spike, slightly increased the thousand grain weight, but significantly increased the seed setting rate of rice, which could effectively improve the yield of rice, and the yield increase was in the range of 4.56%-11.43%. Spraying Se fertilizer could not only raise rice yield to some extent, but also improve the selenium content in rice at heading stage. Spraying Se fertilizer 7 days after full heading stage could significantly increase the yield and selenium content in rice.