Abstract: Bacterial manure as an important biological control approach, was widely used in crop production. This study was conducted to evaluate the effect of two bacterial manures, Effective Microorganisms (EM) and Bacillus mucilaginosus, with different treatments (5 %, 10 % and 15 %) and their combination on the seed germination rate and seedling growth of Honghuadajinyuan and Yunyan 85. The results showed as follows: Adding EM and Bacillus mucilaginosus in seedbed could improve the germination rate of two tobacco seeds. The effect of promote germination to the two tobacco seeds was mainly reflected in the post-germination stage (in 17 ~ 19d) when adding one bacterial fertilizer. Promotion effect was feflected in whole germination stage of Honghuadajinyuan when two bacterial fertilizer as co-addition, while slightly inhibition was mainly reflected of Yunyan 85 in the initial germination stage, the germination rate was higher than the control at 21 d. The treatments of bacterial manures improved the growth of main root and lateral root of Honghuadajinyuan and Yunyan 85 at three leaf period, the promotion of low concentration (5 %) to two tobacco seedings was remarkable when adding EM only. While the promotion effect of middle concentration (10 %) was notable when adding Bacillus mucilaginosus only. The argonomic characteristics also could be improved with the combination of two bacterial fertilizer in three leaf period. The application of EM and Bacillus mucilaginosus promoted the growth of root, the total number of root so as to modify the growth status at five leaf period. The plant height, stem diameter ,longest leaf length and maximum leaf width of two tobacco seedlings were also improved with bacterial manures at 70 d after treated(before transplanting). To sum up, adding EM and Bacillus mucilaginosus in seedbed could improve germination rate and seedling growth of tobacco in different extent, especially adding 5 ~ 10 %.