Abstract: Freezing injury is one of the main factors influencing the growth and development for European hazel. To study the cold-resistance for different European hazel cultivars, artificial low temperature stress was used, and the electrical permeation rates of mature one-year-old shoots of 7 flat-European hazel cultivars were determined during the dormant period. Then combining logistic equation and curve-fitting, semi-lethal low temperature (LT50) and cold- resistance of the 7 cultivars were calculated and appraised, which were compared with the results of field over-wintering test. Results showed that with the fall of temperature, the electrical permeation rate of the 7 cultivars increased, and the changing curves were almost the same. Yet the increasing extent was different. The semi-lethal low temperature (LT50) was-19.224℃ for‘06-1’,-18.942℃ for ‘Davey’, - 16.630℃ for‘82- 11’, - 16.246℃ for‘Jinling’, - 16.139℃ for‘Liaozhen 3’, - 15.859℃ for ‘Kuixiang’and -15.248℃ for‘84-572’, respectively, which showed that the order of cold-resistance of‘06- 21’>‘Davey’>‘82-11’>‘Jinling’>‘Liaozhen 3’>‘Kuixiang’>‘84-572’. This result was almost the same as that of the field test, which indicated that the semi-lethal low temperature (LT50) could be used as an important index for evaluating cold-resistance of flat-European hazel cultivars.