Abstract: The paper aims to promote the development of ecological agriculture, and provide effective countermeasures for prevention and control of agricultural non-point source pollution.. [Method] The authors summaried about prevention and control, demonstration technologies, effects of regional non-point source pollution from aspects such as integrated layout of planting and breeding, reduction of fertilizer and control of chemical pesticide.[Resutl] It put forward 3 main countermeasures for prevention of agricultural non-point source pollution in Lixiahe area, Jiangsu. Firstly, implementing eco-agricultural pattern of green planting-breeding, creating and completing 4 types and 18 kinds of eco-agricultural pattern of green planting-breeding which were based on agriculture and pasture, forestry and grazing, agriculture, forestry and fishery, and “four hydrophilies” (rice, aquatic product, waterfowl and aquatic vegetables), also completing integrated supporting technology system, working out 16 provincial standards and forming technical standard system of green planting-breeding, which were demonstrated and popularized in Lixiahe area, Jiangsu. Secondly, establishing technical system for green-fertilizer based on green planting-breeding. Thirdly, building green technical system for disease pest and weed control on account of green planting-breeding. It has played leading role in accelerating development of local eco-agriculture and demonstration-driven role in realizing prevention of non-point source pollution at root.