Abstract: Aiming at the poor survival rate for twig cutting of cherry dwarf-rootstock‘YT101’and obtaining the optimum formula of rooting regulators, the effects of different rooting regulators with various concentrations and soaking durations on cutting rooting of twigs of cherry dwarf-rootstock‘YT101’were studied. The results showed that the plant growth regulators increased the rooting percentage significantly and the combination of IBA with concentration of 1 g/L was the optimum. The rooting percentage and root length did not demonstrate significant difference after different soaking durations. By increasing the soaking duration, the root number had a slightly reduction after a little increase. Considering the practicability and convenience, it was appropriate to use IBA 1 g/L with soaking duration of five minutes, while the quick dipping could be applied in batch production. Compare with that of‘Gisela 5’and‘Gisela 6’, rooting percentage (96.46%), root number (23.73) and root length (17.71 cm) of cherry dwarf-rootstock‘YT101’were the maximum after the application of IBA 1 g/L with quick dipping.