Total Flavonoids in Maize Seedlings Before and After Drought Stress: Determination with Sodium Nitrite-Aluminum Nitrate Colorimetry

doi:10.11923/j.issn.2095-4050.cjas20190800161

Abstract

Abstract:

To study the effects of drought stress on the total flavonoids content of different maize seedlings, sodium nitrite-aluminum nitrate colorimetric method was used to determine the total flavonoid of 'Xudan 20', 'Zhengdan 958' and 'Yuyu 22' maize seedlings before and after drought stress, and the method was verified with methodology. Results showed that the total flavonoids contents in maize seedlings of the three varieties from high to low was 'Xundan 20', 'Zhengdan 958', 'Yuyu 22' before drought stress, and it changed to be 'Zhengdan 958', 'Xundan 20', 'Yuyu 22' after drought stress. The total flavonoids contents in maize seedlings of the three varieties increased after drought stress, 'Zhengdan 958' increased the most (16.21%), followed by 'Xundan 20' (7.61%), and the lowest was in 'Yuyu 22' (2.67%). The precision, stability and recovery of the sodium nitrite-aluminum nitrate colorimetric method used in the study were conformed to the requirement of methodology, showed good feasibility. Before and after drought stress, the total flavonoids of different varieties had different response due to their distinct sensitivity. This experiment could provide certain references for studying the changes of total flavonoids in secondary metabolites of different maize varieties under drought stress.

Key words: Maize, Drought Stress, Sodium Nitrite-Aluminum Nitrate Colorimetric Method, Total Flavonoids Content

CLC Number:

S132

Ren Yuanyu, Wei Dongwei, Wang Zhongwei, Zhou Yaping. Total Flavonoids in Maize Seedlings Before and After Drought Stress: Determination with Sodium Nitrite-Aluminum Nitrate Colorimetry[J]. Journal of Agriculture, 2020, 10(5): 15-20.

Figures/Tables 8

References 23

[1]	任志强, 王晓清, 卜华虎 , 等. 玉米抗旱育种研究进展[J]. 山西农业科学, 2019,47(7):1291-1294.
[2]	王德信, 杨晓莹 . 玉米幼苗对干旱胁迫的生理响应[J]. 贵州农业科学, 2018,46(4):26-29.
[3]	Ghahfarokhi G, Mansurifar S , Taghizadeh-mehrjardi, et al. Effects of Drought Stress and rewatering on Antioxidant Systems and Relative Water Content in Different Growth Stages of Maize (Zea mays L.) Hybrids[J]. Archives of Agronomy and Soil Science, 2015,61(4/6):493-506.
[4]	韩金龙, 王同燕, 徐子利 , 等. 玉米抗旱机理及抗旱性鉴定指标研究进展[J]. 中国农学通报, 2010,26(21):142-146.
[5]	Freire De Sousa D P, Braga B B, Gondim F A , et al. Increased Drought Tolerance in Maize Plants Induced by H₂O₂ is Closely Related to an Enhanced Enzymatic Antioxidant System and Higher Soluble Protein and Organic Solutes Contents[J]. Theoretical and Experimental Plant Physiology, 2016,28(3):297-306. doi: 10.1007/s40626-016-0069-3 URL
[6]	刘海芳, 魏东伟, 刘全军 , 等. 太行菊不同器官中绿原酸和4种黄酮类物质含量研究[J]. 天然产物研究与开发, 2013,25(5):646-651,671.
[7]	Catarino M D , Alves-Silva J M, Pereira O R, et al. Antioxidant Capacities of Flavones and Benefits in Oxidative-Stress Related Diseases[J]. Current Topics in Medicinal Chemistry, 2015,15(2):105-119. doi: 10.2174/1568026615666141209144506 URL
[8]	赵雪巍, 刘培玉, 刘丹 , 等. 黄酮类化合物的构效关系研究进展[J]. 中草药, 2015,46(21):3264-3271.
[9]	Jayashree B S, Venkatachalam H, Sanchari B M , et al. Flavones and Their Analogues as Bioactive Compounds--An Overview[J]. Mini-Reviews in Organic Chemistry, 2019,16(4):377-391. doi: 10.2174/1570193X15666180418154510 URL
[10]	周亚萍, 魏东伟, 孙武勇 , 等. 高效液相色谱法测定玉米幼苗叶片中4种酚酸类化合物[J]. 中国农学通报, 2019,35(13):36-41.
[11]	Dongyun M, Dexiang S, Chenyang W . Expression of Flavonoid Biosynjournal Genes and Accumulation of Flavonoid in Wheat Leaves in Response to Drought Stress[J]. Plant Physiology and Biochemistry, 2014,3(24):60-66.
[12]	赵贝贝, 叶蕴灵, 王莉 , 等. 银杏类黄酮响应非生物胁迫研究进展[J]. 扬州大学学报:农业与生命科学版, 2018,39(3):106-112.
[13]	郭慧玲, 张亚军 . 紫外-可见分光光度法测定乌灵胶囊总黄酮含量的方法研究[J]. 广州中医药大学学报, 2019,36(7):1074-1079.
[14]	胡秀丽, 李艳辉, 杨海荣 , 等. HSP70可提高干旱高温复合胁迫诱导的玉米叶片抗氧化防护能力[J]. 作物学报, 2010,36(4):636-644. doi: 10.3724/SP.J.1006.2010.00636 URL
[15]	王洪彪, 王鹏辉, 秦嘉驹 , 等. 植物黄酮类化合物的提纯和含量测定方法研究概况[J]. 吉林化工学院学报, 2018,35(7):44-47.
[16]	杨雅欣, 杨芳, 明惠仪 , 等. 比色法测定-枝黄花总黄酮含量的方法学研究[J]. 亚太传统医药, 2017,13(22):17-19.
[17]	王磊, 刘长姣, 吴婕 . 黑米中黄酮类化合物的稳定性分析[J]. 食品研究与开发, 2018,39(3):14-17.
[18]	孙爱清, 张杰道, 万勇善 , 等. 花生干旱胁迫响应基因的数字表达谱分析[J]. 作物学报, 2013,39(6):1045-1053. doi: 10.3724/SP.J.1006.2013.01045 URL
[19]	王向阳, 冀天会, 孙晓娟 , 等. 河南省夏玉米主栽品种抗旱特性研究[J]. 河南农业科学, 2014,43(4):36-39.
[20]	赵霞, 刘诗慧, 张国方 , 等. 16个玉米杂交种的抗旱性评价[J]. 河南农业科学, 2017,46(12):24-28.
[21]	白生文, 汤超, 田京 , 等. 沙棘果渣总黄酮提取工艺及抗氧化活性分析[J]. 食品科学, 2015,36(10):59-64. doi: 10.7506/spkx1002-6630-201510012 URL
[22]	段宙位, 李维国, 窦志浩 , 等. 沉香叶黄酮类化合物的提取及其抗氧化活性[J]. 食品科学, 2015,36(6):45-50. doi: 10.7506/spkx1002-6630-201506009 URL
[23]	Hidalgo M, Sánchez Moreno C , Pascual-Teresa S D. Flavonoid-Flavonoid Interaction and Its Effect on Their Antioxidant Activity[J]. Food Chemistry, 2010,121(3):691-696. doi: 10.1016/j.foodchem.2009.12.097 URL

项目	样品液1	样品液2	样品液3	样品液4	样品液5	样品液6
A₅₀₈/a.u.	0.686	0.712	0.695	0.686	0.645	0.682
总黄酮含量/(mg/g)	51.79	53.91	52.52	51.79	48.46	51.47

项目	样品液1	样品液2	样品液3	样品液4	样品液5	样品液6
A₅₀₈/a.u.	0.686	0.712	0.695	0.686	0.645	0.682
总黄酮含量/(mg/g)	51.79	53.91	52.52	51.79	48.46	51.47

项目	0 h	0.5 h	1 h	1.5 h	2 h	2.5 h	3 h	3.5 h
A₅₀₈/a.u.	0.673	0.671	0.655	0.640	0.640	0.659	0.669	0.707
总黄酮含量/(mg/g)	50.73	50.57	49.27	48.05	48.05	49.59	50.41	53.50

项目	0 h	0.5 h	1 h	1.5 h	2 h	2.5 h	3 h	3.5 h
A₅₀₈/a.u.	0.673	0.671	0.655	0.640	0.640	0.659	0.669	0.707
总黄酮含量/(mg/g)	50.73	50.57	49.27	48.05	48.05	49.59	50.41	53.50

样品		样品测定值/(mg/g)	加标量/(mg/g)	加标样品测定值/(mg/g)	回收率/%	平均回收率/%
干旱组	浚单20	53.50	10	64.63	101.80	102.29
	郑单958	46.67	10	57.72	100.86
	豫玉22	43.17	10	55.12	103.68
对照组	浚单20	48.86	10	60.00	101.93
	郑单958	42.52	10	53.65	102.35
	豫玉22	41.71	10	52.92	102.37