Auricularia of Different Varieties and Habitats: A Comparative Study on the Polysaccharides Content

doi:10.11923/j.issn.2095-4050.cjas2020-0155

Abstract

Abstract:

The paper aims to establish a method for the determination of polysaccharides in Auricularia and compare the polysaccharide content of Auricularia in different varieties and regions. Anthrone-sulfuric acid method was used to determine the content of polysaccharides in Auricularia auricula and Auricularia polytricha from different habitats, and the methodological investigation was conducted. The results showed that: the concentration of dextran had a good linear relationship with its absorbance in the range of 3.27-23.61 μg/mL (R²=0.9993), with an average recovery rate of 101.2% and RSD of 3.5% (n=9). The polysaccharides content of A. auricula from Jiangshan, Benxi and Zhangzhou was 4.1%, 3.0% and 3.5%, respectively; the polysaccharides content of A. polytricha from Jiangshan and Xuzhou was 4.1% and 3.6%, respectively. The method is suitable for the determination of polysaccharides in Auricularia. There was certain difference in the polysaccharides content of A. auricula from different habitats, but not for the A. polytricha. The overall difference in the polysaccharides content between the two varieties was not obvious.

Key words: Auricularia auricular, Auricularia polytricha, Polysaccharides, Anthrone-sulfuric Acid Method, Region

CLC Number:

S184

Li Zhen, Chen Jiemei, Liu Xianzhao, Tan Lirong, Sun Hongmei. Auricularia of Different Varieties and Habitats: A Comparative Study on the Polysaccharides Content[J]. Journal of Agriculture, 2021, 11(3): 25-29.

Figures/Tables 5

References 27

[1]	冯小飞, 陈玉惠, 赵宁, 等. 不同产地黑木耳水溶性多糖与蛋白质含量测定比较[J]. 食用菌, 2016(4):57-58.
[2]	吕文英. 黑木耳和毛木耳中无机营养元素含量的测定与研究[J]. 微量元素与健康研究, 2007,24(4):30-31.
[3]	李睿瑞, 唐金敏, 赵行建, 等. 黑木耳中水溶性多糖提取条件的研究[J]. 安徽农学通报, 2018,24(11):9-10.
[4]	Yoona S J, Yub M A, Pyunb Y R, et al. The nontoxic mushroom Auricularia auricular contains polysaccharide with anticoagulant activity mediated by antithrombin[J]. Thrombosis Research, 2003,112(3):151-158. doi: 10.1016/j.thromres.2003.10.022 URL pmid: 14967412
[5]	Zeng W C, Zhang Z, Gao H, et al. Characterization of antioxidant polysaccharides from Auricularia auricular using microwave - assisted extraction[J]. Carbohydrate Polymers, 2012,89(2):694-700. doi: 10.1016/j.carbpol.2012.03.078 URL pmid: 24750775
[6]	Chen Z Q, Wang J H, Fan Z L, et al. Effects of polysaccharide from the fruiting bodies of Auricularia auricular on glucose metabolism in⁶⁰Co-γ-radiated mice [J]. International Journal of Biological Macromolecules, 2019,135:887-897. doi: 10.1016/j.ijbiomac.2019.05.136 URL pmid: 31132438
[7]	Nguyen T L, Chen J, Hu Y L, et al. In vitro antiviral activity of sulfated Auricularia auricula polysaccharides[J]. Carbohydrate Polymers, 2012,90(3):1254-1258. doi: 10.1016/j.carbpol.2012.06.060 URL pmid: 22939338
[8]	Reza M A, Hossain M A, Lee S J, et al. Dichlormethane extract of the jelly ear mushroom Auricularia auricular Judae (higher Basidiomycetes) inhibits tumor cell growth in vitro[J]. International Journal of Medicinal Mushrooms, 2014,16(1):37-47. doi: 10.1615/intjmedmushr.v16.i1.40 URL pmid: 24940903
[9]	Song G L, Du Q Z. Structure characterization and antitumor activity of an α β-glucan polysaccharide from Auricularia polytricha[J]. Food Research International, 2012,45(1):381-387. doi: 10.1016/j.foodres.2011.10.035 URL
[10]	Lu A X, Yu M G, Shen M, et al. Preparation of the Auricularia auricular polysaccharides simulated hydrolysates and their hypoglycaemic effect[J]. International Journal of Biological Macromolecules, 2018,106:1139-1145. URL pmid: 28847604
[11]	Yang B K, Ha J Y, Jeong S C, et al. Hypolipidemic effect of an exo-biopolymer produced from submerged mycelial culture of Auricularia polytricha in rats[J]. Biotechnology Letters, 2002,24(16):1319-1325. doi: 10.1023/A:1019831929570 URL
[12]	Ma J W, Qiao Z Y, Xiang X. Optimisation of extraction procedure for black fungus polysaccharides and effect of the polysaccharides on blood lipid and myocardium antioxidant enzymes activities[J]. Carbohydrate Polymers, 2011,84(3):1061-1068. doi: 10.1016/j.carbpol.2010.12.068 URL
[13]	Zhao S, Rong C, Liu Y, et al. Extraction of a soluble polysaccharide from Auricularia polytricha and evaluation of its anti-hypercholesterolemic effect in rats[J]. Carbohydrate Polymers, 2015,122:39-45. doi: 10.1016/j.carbpol.2014.12.041 URL pmid: 25817640
[14]	Nguyen T L, Wang D, Hu Y, et al. Immuno-enhancing activity of sulfated Auricularia auricula polysaccharides[J]. Carbohydrate Polymers, 2012,89(4):1117-1122. doi: 10.1016/j.carbpol.2012.03.082 URL pmid: 24750922
[15]	杨伟君, 马诗经, 焦春伟, 等. 灵芝孢子粉多糖含量检测方法的优化[J]. 中国食用菌, 2018,37(2):67-72.
[16]	殷贝贝, 赵爽, 刘宇, 等. 市售不同品种木耳蛋白质和水溶性多糖含量的研究[J]. 北方园艺, 2011(23):147-149.
[17]	杜娟, 时文静. 香菇、金针菇、黑木耳多糖的提取与测定[J]. 江苏农业科学, 2016,44(8):347-350.
[18]	郭晓雷, 朱思潮. 硫酸蒽酮法与硫酸苯酚法测定灵芝多糖含量比较[J]. 中国中医药学刊, 2010,28(9):2000-2002.
[19]	颜梦秋, 唐庆九, 杨焱, 等. 醇沉条件和干燥方式对毛头鬼伞子实体多糖品质的影响[J]. 菌物研究, 2014,12(4):221-225.
[20]	耿安静, 陈健. 香菇精多糖乙醇沉淀规律的研究[J]. 食品科学, 2010,31(4):99-102. doi: 10.7506/spkx1002-6300-201004022 URL
[21]	Xue B L, Wen J L, Xu F, et al. Structural characterization of hemicelluloses fractionated by graded ethanol precipitation from Pinus yunnanensis[J]. Carbohydrate Research, 2012,352(9):159-165. doi: 10.1016/j.carres.2012.02.004 URL
[22]	Balto A S, Lapis T J, Silver R K, et al. On the use of differential solubility in aqueous ethanol solutions to narrow the DP range of food-grade starch hydrolysis products[J]. Food Chemistry, 2016,197:872-880. doi: 10.1016/j.foodchem.2015.10.120 URL pmid: 26617029
[23]	黄庆斌, 郑志昌, 陈竟豪, 等. 不同醇沉竹荪多糖组分对青春双歧杆菌体外增殖作用的影响[J]. 食品工业科技, 2020(4):86.
[24]	孙晓燕, 蔡昌利, 徐丽莉, 等. 多糖含量测定方法的比较[J]. 现代中药研究与实践, 2015,29(3):58-62.
[25]	庄伟, 屈咪, 赵迪, 等. 黑木耳多糖的结构组成及其免疫活性研究[J]. 食品科技, 2020,45(2):205-210.
[26]	李承范. 7种常见食用菌中多糖的提取和测定[J]. 光谱实验室, 2012,29(2):996-999.
[27]	周勇, 易延逵, 杨晓敏, 等. 香菇中多糖含量测定方法的比较研究[J]. 食品研究与开发, 2016,37(13):124-128.

样本号	本底量/μg	加入量/μg	吸光度/Abs	实测值/μg	回收率/%	平均值/%	RSD/%
50%-1	356.51	153.45	0.272	502.07	94.86	101.2	3.5
50%-2	337.92	153.45	0.268	494.55	102.07
50%-3	365.80	153.45	0.278	513.35	96.15
100%-1	349.07	306.90	0.361	669.36	104.36
100%-2	341.64	306.90	0.354	656.20	102.50
100%-3	334.20	306.90	0.349	646.80	101.86
150%-1	352.79	460.35	0.449	834.77	104.70
150%-2	337.92	460.35	0.431	800.94	100.58
150%-3	343.49	460.35	0.442	821.62	103.86

样本号	本底量/μg	加入量/μg	吸光度/Abs	实测值/μg	回收率/%	平均值/%	RSD/%
50%-1	356.51	153.45	0.272	502.07	94.86	101.2	3.5
50%-2	337.92	153.45	0.268	494.55	102.07
50%-3	365.80	153.45	0.278	513.35	96.15
100%-1	349.07	306.90	0.361	669.36	104.36
100%-2	341.64	306.90	0.354	656.20	102.50
100%-3	334.20	306.90	0.349	646.80	101.86
150%-1	352.79	460.35	0.449	834.77	104.70
150%-2	337.92	460.35	0.431	800.94	100.58
150%-3	343.49	460.35	0.442	821.62	103.86

品种	产地	各批次多糖含量										含量平均值
品种	产地	S1	S3	S3	S4	S5	S6	S7	S8	S9	S10	含量平均值
黑木耳	浙江江山	3.9	3.1	4.6	6.3	4.4	4.9	4.5	3.2	2.9	3.4	4.1A
黑木耳	福建漳州	3.3	3.5	3.2	3.3	4.0	3.1	3.5	3.9	3.1	4.0	3.5A
黑木耳	辽宁本溪	2.8	3.6	3.3	3.3	3.0	3.0	3.4	2.6	2.5	2.7	3.0B
毛木耳	浙江江山	4.6	3.7	4.0	4.0	5.8	3.7	3.4	4.3	3.5	4.1	4.1A
毛木耳	江苏徐州	3.3	3.9	3.9	3.4	3.5	3.2	4.4	3.4	3.5	3.5	3.6A

品种	产地	各批次多糖含量										含量平均值
品种	产地	S1	S3	S3	S4	S5	S6	S7	S8	S9	S10	含量平均值
黑木耳	浙江江山	3.9	3.1	4.6	6.3	4.4	4.9	4.5	3.2	2.9	3.4	4.1A
黑木耳	福建漳州	3.3	3.5	3.2	3.3	4.0	3.1	3.5	3.9	3.1	4.0	3.5A
黑木耳	辽宁本溪	2.8	3.6	3.3	3.3	3.0	3.0	3.4	2.6	2.5	2.7	3.0B
毛木耳	浙江江山	4.6	3.7	4.0	4.0	5.8	3.7	3.4	4.3	3.5	4.1	4.1A
毛木耳	江苏徐州	3.3	3.9	3.9	3.4	3.5	3.2	4.4	3.4	3.5	3.5	3.6A