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Journal of Agriculture ›› 2021, Vol. 11 ›› Issue (7): 84-91.doi: 10.11923/j.issn.2095-4050.cjas2020-0115

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An Autotoxicity p-Hydroxybenzoic Acid-degrading Strain ZH2: Isolation and Application

Xiao Rong1(), Deng Shu1, Zhao Jing2, Zhang Chunfen1, Nie Yuanjun3, Cao Qiufen2()   

  1. 1Pomology Institute, Shanxi Agricultural University, Taiyuan 030031, Shanxi, China
    2College of Life Sciences, Shanxi Agricultural University, Taiyuan 030031, Shanxi, China
    3College of Economics and Management, Shanxi Agricultural University, Taiyuan 030031, Shanxi, China
  • Received:2020-07-01 Revised:2020-09-09 Online:2021-07-20 Published:2021-08-04
  • Contact: Cao Qiufen E-mail:xiaorongzhujian@126.com;qiufengcao@163.com

Abstract:

Autotoxicity is the main factor causing obstacles to continuous cropping plants. The aim of the study is to screen bacteria from soil which can degrade autotoxicity. A selective screening method was used to screen p-Hydroxybenzoic acid (PHBA) degradation bacterium. Bacterial species were identified by combining the morphological characteristics, physiological and biochemical characteristics and 16S rRNA sequencing results. The ability of the strain to degrade PHBA was determined by ultraviolet spectrophotometry. The effect of detoxification was verified by a pot experiment of cucumber. The results showed that one degradation strain (No. ZH2) was isolated and identified as Pseudomonas chlororaphis. On the condition of pure culture, ZH2 could degrade 97% at 72 h under 5 mg/mL PHBA concentration. In potted conditions, when the concentration of PHBA in the substrate was 10 mg/g, ZH2 could effectively alleviate the growth inhibition of PHBA on cucumber. We report a Pseudomonas chlororaphis strain screened from soil can degrade PHBA, it has the potential value of controlling continuous cropping obstacles.

Key words: p-Hydroxybenzoic Acid, Autotoxicity, Pseudomonas chlororaphis, Continuous Cropping Obstacle, Degradation

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