New Sweet Potato Germplasm Created by Radiation Mutation Technology

doi:10.11923/j.issn.2095-4050.cjas2021-0001

Abstract

Abstract:

The purpose of the study is to obtain beneficial mutants of sweetpotato by radiation mutation technology, and enrich sweetpotato germplasm resources. In this experiment, the main sweetpotato variety ‘Shangshu 19’ was used as test material. The embryogenic suspension cells were irradiated with⁶⁰Co γ ray and the irradiation doses was 80 Gy. After 6 weeks of irradiation, the surviving cells were transferred into MS solid medium containing 1.0 mg/L ABA to induce differentiation, and then transferred into MS medium to obtain 138 complete plants. Through investigating and determining the top leaf color, vein color, top leaf shape, leaf shape, top villi, potato shape, drying rate and soluble sugar content of the irradiated progenies, it was found that the top leaf color of one line (‘S19Y038’) changed, and the drying rate of three lines (‘S19Y116’, ‘S19Y215’and ‘S19Y219’) were increased significantly by 5.98%, 5.65% and 3.32%, respectively. The soluble sugar content of one strain (‘S19Y013’) was significantly increased by 17.81% compared with that of the control. These mutants with excellent characters could enrich the sweetpotato germplasm resources and have a good application prospect in breeding high-quality sweetpotato varieties.

Key words: Radiation Mutagenesis, Screening, Sweetpotato, Drying Rate, Soluble Sugar Content, New Germplasm

CLC Number:

S531

Jie Qin, Tao Chunlai, Zhang Gong, Cui Shaoyu, Shi Ying, Liu Yang, Zhou Jiandong, Du Deyu. New Sweet Potato Germplasm Created by Radiation Mutation Technology[J]. Journal of Agriculture, 2021, 11(8): 22-26.

Figures/Tables 9

References 21

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性状	商薯19(CK)	S19Y038
顶芽色	绿	浅绿
顶叶型	凹	平
顶叶形	尖心	缺刻
顶叶缺刻深浅	无	浅
顶叶缺刻类型	无	单缺刻
顶叶色	褐绿	浅绿
脉基色	浅绿	浅紫
柄基色	绿	浅绿

性状	商薯19(CK)	S19Y038
顶芽色	绿	浅绿
顶叶型	凹	平
顶叶形	尖心	缺刻
顶叶缺刻深浅	无	浅
顶叶缺刻类型	无	单缺刻
顶叶色	褐绿	浅绿
脉基色	浅绿	浅紫
柄基色	绿	浅绿

样品	烘干率/%	样品	烘干率/%
商薯19(CK)	30.10	S19Y027	29.50
S19Y013	25.40	S19Y028	29.00
S19Y016	27.90	S19Y211	27.00
S19Y019	30.30	S19Y212	27.50
S19Y011	29.90	S19Y213	27.30
S19Y110	28.90	S19Y215	31.80
S19Y111	26.30	S19Y216	26.90
S19Y113	27.00	S19Y218	28.30
S19Y116	31.90	S19Y219	31.10
S19Y021	27.80	S19Y220	27.30
S19Y022	27.90	S19Y292	28.20
S19Y023	28.90	S19Y031	29.90
S19Y026	29.40	S19Y034	27.00

样品	烘干率/%	样品	烘干率/%
商薯19(CK)	30.10	S19Y027	29.50
S19Y013	25.40	S19Y028	29.00
S19Y016	27.90	S19Y211	27.00
S19Y019	30.30	S19Y212	27.50
S19Y011	29.90	S19Y213	27.30
S19Y110	28.90	S19Y215	31.80
S19Y111	26.30	S19Y216	26.90
S19Y113	27.00	S19Y218	28.30
S19Y116	31.90	S19Y219	31.10
S19Y021	27.80	S19Y220	27.30
S19Y022	27.90	S19Y292	28.20
S19Y023	28.90	S19Y031	29.90
S19Y026	29.40	S19Y034	27.00

		方差方程的Levene检验		均值方程的t检验
		F	Sig.	t	df	Sig. （双侧）	均值差值	标准误差值	差分的95%置信区间
		F	Sig.	t	df	Sig. （双侧）	均值差值	标准误差值	下限	上限
干物重 S19CK/S19Y116	假设方差相等	1.850	0.245	-11.950	4	0.000	-1.81667	0.15202	-2.23875	-1.39458
干物重 S19CK/S19Y116	假设方差不相等			-11.950	2.423	0.003	-1.81667	0.15202	-2.37265	-1.26069
干物重 S19CK/S19Y219	假设方差相等	3.955	0.118	-4.239	4	0.013	-1.28333	0.30273	-2.12384	-0.44282
干物重 S19CK/S19Y219	假设方差不相等			-4.239	2.100	0.047	-1.28333	0.30273	-2.52828	-0.03839
干物重 S19CK/S19Y215	假设方差相等	0.222	0.662	-22.870	4	0.000	-1.73333	0.07579	-1.94377	-1.52290
干物重 S19CK/S19Y215	假设方差不相等			-22.870	3.811	0.000	-1.73333	0.07579	-1.94794	-1.51873