Polysaccharide Extraction in Lactuca Satya L. and Its Antihyperlipidemic Activity

doi:10.11923/j.issn.2095-4050.cjas20191000234

Abstract

Abstract:

The aim is to establish and optimize the extraction process of polysaccharides from Lactuca Satya L. and to explore the in vitro hypolipidemic activity of the polysaccharides. Lactuca Satya L. was used as material, polysaccharides was extracted by water/ethanol method. The polysaccharide extraction process of Lactuca Satya L. was established by back propagation (BP) neural network and orthogonal design. The in vitro hypolipidemie of polysaccharide in Lactuca Satya L. was studied by in vitro binding of cholates. The optimal condition for Lactuca Satya L. polysaccharide extraction was at 89℃ with solvent amount of 44 times in 1.6 h and 4 extracting times. The polysaccharides in Lactuca Satya L. and their alcohol-precipitated fractions had better cholate binding capacity with sodium taurocholate (STC), glychocholic acid sodium salts (SGC), sodium cholate (SC). BP neural network combined with orthogonal test could reduce experiment times effectively, analyze the change rules of Lactuca Satya L. polysaccharide extraction factors, and obtain the optimal extraction parameters. The hypolipidemic mechanism of Lactuca Satya L. polysaccharide might be the combination of cholic acids in intestine, which affected the lipids absorption.

Key words: Lactuca Satya L., Polysaccharide, extraction process, Orthogonal Design, BP Neural Network, Hypolipidemic

CLC Number:

Yang Fengrong, Gao Jianping, Li Chunyan. Polysaccharide Extraction in Lactuca Satya L. and Its Antihyperlipidemic Activity[J]. Journal of Agriculture, 2021, 11(11): 81-87.

Figures/Tables 14

References 26

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水平	因素
水平	A提取温度/℃	B提取时间/h	C提取次数/次	D溶剂倍量/倍
1	60	0.5	2	40
2	70	1	3	50
3	80	1.5	4	60

水平	因素
水平	A提取温度/℃	B提取时间/h	C提取次数/次	D溶剂倍量/倍
1	60	0.5	2	40
2	70	1	3	50
3	80	1.5	4	60

编号	因素				多糖得率/%
编号	A提取温度	B提取时间	C提取次数	D溶剂倍量	多糖得率/%
1	1(60℃)	1(0.5 h)	1(2)	1(40)	6.5
2	1(60℃)	2(1 h)	2(3)	2(50)	9.9
3	1(60℃)	3(1.5 h)	3(4)	3(60)	10.3
4	2(70℃)	1(0.5 h)	3(4)	2(50)	10.2
5	2(70℃)	2(1 h)	1(2)	3(60)	9.8
6	2(70℃)	3(1.5 h)	2(3)	1(40)	8.8
7	3(80℃)	1(0.5 h)	2(3)	3(60)	9.4
8	3(80℃)	2(1 h)	3(4)	1(40)	8.5
9	3(80℃)	3(1.5 h)	1(2)	2(50)	10.1
K1	8.900	8.700	7.933	8.800
K2	9.600	9.400	10.067	9.367
K3	9.333	9.733	9.833	9.667
R	0.700	1.033	2.134	0.867

编号	因素				多糖得率/%
编号	A提取温度	B提取时间	C提取次数	D溶剂倍量	多糖得率/%
1	1(60℃)	1(0.5 h)	1(2)	1(40)	6.5
2	1(60℃)	2(1 h)	2(3)	2(50)	9.9
3	1(60℃)	3(1.5 h)	3(4)	3(60)	10.3
4	2(70℃)	1(0.5 h)	3(4)	2(50)	10.2
5	2(70℃)	2(1 h)	1(2)	3(60)	9.8
6	2(70℃)	3(1.5 h)	2(3)	1(40)	8.8
7	3(80℃)	1(0.5 h)	2(3)	3(60)	9.4
8	3(80℃)	2(1 h)	3(4)	1(40)	8.5
9	3(80℃)	3(1.5 h)	1(2)	2(50)	10.1
K1	8.900	8.700	7.933	8.800
K2	9.600	9.400	10.067	9.367
K3	9.333	9.733	9.833	9.667
R	0.700	1.033	2.134	0.867

因素	偏差平方和	自由度	F比	F临界值	显著性
提取温度	0.749	2	0.784	6.940
提取时间	1.669	2	1.747	6.940
提取次数	8.216	2	8.599	6.940	P<0.05^*
溶剂倍量	1.162	2	1.216	6.940
误差	1.91	4