Journal of Agriculture ›› 2021, Vol. 11 ›› Issue (11): 88-97.doi: 10.11923/j.issn.2095-4050.cjas2020-0152
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Chen Zihan(), Ren Jianguo, Wang Junli()
Received:
2020-07-30
Revised:
2020-10-19
Online:
2021-11-20
Published:
2021-12-09
Contact:
Wang Junli
E-mail:577908271@qq.com;411395583@qq.com
CLC Number:
Chen Zihan, Ren Jianguo, Wang Junli. Research Advances on Plant DNA Methylation[J]. Journal of Agriculture, 2021, 11(11): 88-97.
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URL: http://nxxb.caass.org.cn/EN/10.11923/j.issn.2095-4050.cjas2020-0152
方法 | 基本原理 | 主要覆盖范围 | 是否需要参考基因 | 参考文献 |
---|---|---|---|---|
高效液相色谱 | 水解、紫外检测 | 全基因组 | 否 | [ |
甲基化敏感扩增多态性 | 限制性内切酶 | 高CG区域 | 否 | [ |
全基因组亚硫酸盐测序 | 亚硫酸盐处理 | 全基因组 | 是 | [ |
简并代表性亚硫酸氢盐测序技术 | 亚硫酸盐处理+限制性内切酶 | 启动子和CpG岛 | 是 | [ |
甲基化DNA免疫共沉淀测序技术 | 抗体富集 | 高CG区域 | 是 | [ |
方法 | 基本原理 | 主要覆盖范围 | 是否需要参考基因 | 参考文献 |
---|---|---|---|---|
高效液相色谱 | 水解、紫外检测 | 全基因组 | 否 | [ |
甲基化敏感扩增多态性 | 限制性内切酶 | 高CG区域 | 否 | [ |
全基因组亚硫酸盐测序 | 亚硫酸盐处理 | 全基因组 | 是 | [ |
简并代表性亚硫酸氢盐测序技术 | 亚硫酸盐处理+限制性内切酶 | 启动子和CpG岛 | 是 | [ |
甲基化DNA免疫共沉淀测序技术 | 抗体富集 | 高CG区域 | 是 | [ |
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