Establishment of Droplet Digital PCR Detection Method of Candidatus Liberibacter asiaticus

doi:10.11923/j.issn.2095-4050.cjas2023-0040

Abstract

Abstract:

Huanglongbing disease is a devastating disease of citrus industry in the world. Diseased seedlings, scions and field diseased plants are the primary infection sources of the disease. The establishment of a set of ultra-sensitive detection method for pathogen is important for the early warning and prevention of Huanglongbing disease. In this study, specific primers were designed according to the 16S rDNA gene sequence of Candidatus Liberibacter asiaticus, and a detection method based on droplet digital PCR technology was explored and established, and the sensitivity and detection accuracy of the method was evaluated. The test results showed that the microdroplet digital PCR detection method for Huanglongbing disease established in this study had strong specificity and high sensitivity, and its detection sensitivity was 10 times that of fluorescence quantitative PCR. The microdroplet digital PCR detection technology established in this study provides a new method for the early detection of Huanglongbing disease in citrus seedlings.

Key words: citrus, Candidatus Liberibacter asiaticus, droplet digital PCR, early detection, citrus seedlings

SONG Xiaobing, HUANG Feng, CUI Yiping, PENG Aitian, YUE Maofeng. Establishment of Droplet Digital PCR Detection Method of Candidatus Liberibacter asiaticus[J]. Journal of Agriculture, 2024, 14(1): 39-43.

Figures/Tables 3

References 28

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