Optimization and Application of Efficient Detection System for Extraction of Polysaccharides in Pumpkin

doi:10.11923/j.issn.2095-4050.cjas2025-0019

Abstract

Abstract:

To simplify the determination methods of pumpkin polysaccharide content in breeding process, the optimized experiments of phenol sulfuric acid method after hot water extraction of pumpkin polysaccharide were carried out. The detection wavelength and H₂SO₄ amount were determined by single factor experiment using 23S18 and other pumpkin flesh. The correlation of determination results between unpurified and purified polysaccharide content was clarified. The results showed that the wavelength of pumpkin polysaccharide determined by phenol-sulfuric acid method was 490nm, and the sample in reaction system included: 5% phenol: H₂SO₄ was 2:2:7. The regression equation was established as y=0.0061x+0.1476 (R²=0.9965) and the linear relationship was good when the absorbance was in the range of 0.084-3.684. There was a significant correlation between the unpurified and purified polysaccharide content. The polysaccharide contents of 53 pumpkin parents and combinations were determined and analyzed. Two parents (23S2 and 23S14) and two combinations (23FV26 and 23FV5) with high polysaccharide content of more than 100mg/g were selected for breeding. The efficient detection system for polysaccharides was simple to operate, wide in linear range, and the determination results are significantly related to the results of purified polysaccharide, which would improve the efficiency of pumpkin polysaccharide breeding.

Key words: pumpkin, polysaccharides, single factor experiment, phenol-sulfuric acid method, breeding

SONG Hui, AN Xuejun, HUANG Yunping, ZHANG Xiangqin, LV Yan, QIAN Xinxin. Optimization and Application of Efficient Detection System for Extraction of Polysaccharides in Pumpkin[J]. Journal of Agriculture, 2025, 15(6): 1-9.

Figures/Tables 10

References 40

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编号	类型	多糖含量	编号	类型	多糖含量
23S1	板栗	87.22±0.76bcde	23S10	长条橄榄	60.30±4.18fg
23S2	板栗	108.87±2.19ab	23S11	长条橄榄	89.42±4.69bcd
23S3	板栗	51.55±2.67g	23S12	长条橄榄	80.37±6.66cdef
23S4	板栗	61.85±4.62efg	23S13	小甘龙	87.68±1.06bcde
23S5	板栗	88.54±15.50bcde	23S14	大青圆	115.75±1.39a
23S6	板栗	78.46±7.67cdef	23S15	红皮	55.87±2.79fg
23S7	贝贝	63.91±5.47defg	23S16	红皮	68.05±7.78cdefg
23S8	贝贝	49.61±7.76g	23S17	红皮	68.51±3.09cdefg
23S9	贝贝	92.06±3.24bc	23S18	中印红皮	79.64±7.38cdef

编号	类型	多糖含量	编号	类型	多糖含量
23S1	板栗	87.22±0.76bcde	23S10	长条橄榄	60.30±4.18fg
23S2	板栗	108.87±2.19ab	23S11	长条橄榄	89.42±4.69bcd
23S3	板栗	51.55±2.67g	23S12	长条橄榄	80.37±6.66cdef
23S4	板栗	61.85±4.62efg	23S13	小甘龙	87.68±1.06bcde
23S5	板栗	88.54±15.50bcde	23S14	大青圆	115.75±1.39a
23S6	板栗	78.46±7.67cdef	23S15	红皮	55.87±2.79fg
23S7	贝贝	63.91±5.47defg	23S16	红皮	68.05±7.78cdefg
23S8	贝贝	49.61±7.76g	23S17	红皮	68.51±3.09cdefg
23S9	贝贝	92.06±3.24bc	23S18	中印红皮	79.64±7.38cdef

编号	类型	多糖含量	编号	类型	多糖含量
23FV1	板栗	59.52±0.41defg	23FV19	红皮	86.18±5.90abc
23FV2	板栗	81.50±7.50bcde	23FV20	红皮	62.22±3.15cdefg
23FV3	板栗	45.43±0.78ghi	23FV21	红皮	64.36±5.56bcdefg
23FV4	板栗	76.29±0.73bcdef	23FV22	红皮	63.18±1.94cdefg
23FV5	板栗	102.94±5.01a	23FV23	红皮	52.98±1.05fghi
23FV6	板栗	64.54±1.70bcdefg	23FV24	红皮	62.94±1.22cdefg
23FV7	板栗	83.66±1.33abcd	23FV25	红皮	63.10±0.83cdefg
23FV8	板栗	79.88±1.51bcde	23FV26	红皮	102.61±5.91a
23FV9	板栗	75.89±2.27bcdef	23FV27	其他	47.62±4.29ghi
23FV10	贝贝	68.74±2.33bcdefg	23FV28	红皮	57.04±3.70efgh
23FV11	贝贝	68.78±7.00bcdefg	23FV29	其他	35.09±3.51i
23FV12	贝贝	88.40±1.77ab	23FV30	其他	39.95±2.24hj
23FV13	贝贝	80.58±2.16bcde	23FV31	其他	68.61±1.34bcdefg
23FV14	贝贝	83.64±2.10abcd	23FV32	其他	63.05±4.58cdefg
23FV15	贝贝	80.14±0.70bcde	23FV33	其他	66.23±1.63bcdefg
23FV16	橄榄	76.90±7.38bcde	23FV34	锦栗南瓜	73.18±2.43bcdef
23FV17	橄榄	79.01±3.31bcde	23FV35	贝栗4号	78.69±0.29bcde
23FV18	橄榄	77.29±3.50bcde

编号	类型	多糖含量	编号	类型	多糖含量
23FV1	板栗	59.52±0.41defg	23FV19	红皮	86.18±5.90abc
23FV2	板栗	81.50±7.50bcde	23FV20	红皮	62.22±3.15cdefg
23FV3	板栗	45.43±0.78ghi	23FV21	红皮	64.36±5.56bcdefg
23FV4	板栗	76.29±0.73bcdef	23FV22	红皮	63.18±1.94cdefg
23FV5	板栗	102.94±5.01a	23FV23	红皮	52.98±1.05fghi
23FV6	板栗	64.54±1.70bcdefg	23FV24	红皮	62.94±1.22cdefg
23FV7	板栗	83.66±1.33abcd	23FV25	红皮	63.10±0.83cdefg
23FV8	板栗	79.88±1.51bcde	23FV26	红皮	102.61±5.91a
23FV9	板栗	75.89±2.27bcdef	23FV27	其他	47.62±4.29ghi
23FV10	贝贝	68.74±2.33bcdefg	23FV28	红皮	57.04±3.70efgh
23FV11	贝贝	68.78±7.00bcdefg	23FV29	其他	35.09±3.51i
23FV12	贝贝	88.40±1.77ab	23FV30	其他	39.95±2.24hj
23FV13	贝贝	80.58±2.16bcde	23FV31	其他	68.61±1.34bcdefg
23FV14	贝贝	83.64±2.10abcd	23FV32	其他	63.05±4.58cdefg
23FV15	贝贝	80.14±0.70bcde	23FV33	其他	66.23±1.63bcdefg
23FV16	橄榄	76.90±7.38bcde	23FV34	锦栗南瓜	73.18±2.43bcdef
23FV17	橄榄	79.01±3.31bcde	23FV35	贝栗4号	78.69±0.29bcde
23FV18	橄榄	77.29±3.50bcde

仪器	用量	23FV5	23FV12	23FV19	24S8	24S17
分光光度计	1 mL	0.078±0.000e	0.060±0.001e	0.046±0.002e	0.036±0.001e	0.038±0.001e
	3 mL	1.218±0.000d	0.991±0.004d	1.142±0.005d	0.165±0.001d	0.305±0.003d
	5 mL	2.578±0.035c	2.241±0.011c	2.459±0.013c	0.852±0.001c	1.486±0.012c
	7 mL	2.699±0.043b	2.701±0.010a	2.7±0.003a	1.192±0.001a	2.017±0.006a
	9 mL	2.673±0.019b	2.371±0.006b	2.546±0.017b	0.98±0.002b	1.637±0.005b
多功能微孔板检测仪	100 μL	0.063±0.001e	0.06±0.001e	0.06±0.002e	0.05±0.001e	0.08±0.001e
	300 μL	0.478±0.001d	0.331±0.00d	0.378±0.002d	0.123±0.001d	0.196±0.001d
	500 μL	1.651±0.009c	1.291±0.01c	1.528±0.006c	0.492±0.001c	0.845±0.010c
	700 μL	2.847±0.018a	2.203±0.004a	2.373±0.01a	0.737±0.002a	1.22±0.008a
	900 μL	2.349±0.008b	1.534±0.012b	1.802±0.003b	0.602±0.006b	0.99±0.002b