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Journal of Agriculture ›› 2024, Vol. 14 ›› Issue (3): 21-27.doi: 10.11923/j.issn.2095-4050.cjas2023-0041

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High-throughput Detection System for Carotenoids: Establishment and Application in Pumpkin

SONG Hui(), AN Xuejun, HUANG Yunping, ZHANG Xiangqin   

  1. Ningbo Academy of Agricultural Sciences, Ningbo 345040, Zhejiang, China
  • Received:2023-02-21 Revised:2023-05-15 Online:2024-03-16 Published:2024-03-16


In order to establish the high-throughput system of extraction and detection for carotenoids in pumpkin, the pumpkin flesh was used as material, the optimum extraction solution, temperature and material-liquid ratio were determined and optimized by single factor experiments and L27(313) orthogonal experimental design, respectively. The results showed that ethanol was the best extraction solution for extracting carotenoids from pumpkin. The pumpkin pulp was extracted with material-ethanol liquidratio of 1:28at 55℃ for 90min, and the yield of carotenoids was significantly higher [(7.56±0.14) mg/100 g] than other combinations. Among the extraction conditions of carotenoids, temperature showed the greatest influence on extraction efficiency, followed by the interaction of material-liquid ratio × extraction time and extraction time × temperature. The carotenoid contents of 23 different types of pumpkin were detected by high-throughput extraction and detection system. The Cucurbita maximam variety of ‘Beili No.4’and the C. moschata × C. maximam inbred line of 20F8 showed the significantly higher carotenoid contents [(31.40±0.35) mg/100 g] and (24.72±0.72) mg/100 g, respectively)] than other tested pumpkin, which could be used for cultivating new pumpkin varieties rich in carotenoids. The efficient detection system for carotenoids content in pumpkin was labor-saving and accurate, which would improve the efficiency of pumpkin nutrition quality breeding.

Key words: pumpkin, carotenoids, high-throughput detection system, distant hybridization, nutrition quality breeding