High-throughput Detection System for Carotenoids: Establishment and Application in Pumpkin

doi:10.11923/j.issn.2095-4050.cjas2023-0041

Abstract

Abstract:

In order to establish the high-throughput system of extraction and detection for carotenoids in pumpkin, the pumpkin flesh was used as material, the optimum extraction solution, temperature and material-liquid ratio were determined and optimized by single factor experiments and L₂₇(3¹³) orthogonal experimental design, respectively. The results showed that ethanol was the best extraction solution for extracting carotenoids from pumpkin. The pumpkin pulp was extracted with material-ethanol liquidratio of 1:28at 55℃ for 90min, and the yield of carotenoids was significantly higher [(7.56±0.14) mg/100 g] than other combinations. Among the extraction conditions of carotenoids, temperature showed the greatest influence on extraction efficiency, followed by the interaction of material-liquid ratio × extraction time and extraction time × temperature. The carotenoid contents of 23 different types of pumpkin were detected by high-throughput extraction and detection system. The Cucurbita maximam variety of ‘Beili No.4’and the C. moschata × C. maximam inbred line of 20F8 showed the significantly higher carotenoid contents [(31.40±0.35) mg/100 g] and (24.72±0.72) mg/100 g, respectively)] than other tested pumpkin, which could be used for cultivating new pumpkin varieties rich in carotenoids. The efficient detection system for carotenoids content in pumpkin was labor-saving and accurate, which would improve the efficiency of pumpkin nutrition quality breeding.

Key words: pumpkin, carotenoids, high-throughput detection system, distant hybridization, nutrition quality breeding

SONG Hui, AN Xuejun, HUANG Yunping, ZHANG Xiangqin. High-throughput Detection System for Carotenoids: Establishment and Application in Pumpkin[J]. Journal of Agriculture, 2024, 14(3): 21-27.

Figures/Tables 9

References 26

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代号	商品名	类型	编号	类胡萝卜素
20F1	-	中印自交系	1	5.12±0.34	bc
20F2	-	中印自交系	2	7.04±0.46	de
20F3	-	中印自交系	3	5.62±0.19	cd
20F4	-	中印自交系	4	4.93±0.17	bc
20F5	-	中印自交系	5	6.70±0.78	de
20F6	-	中印自交系	6	6.44±0.28	cde
20F7	-	中印自交系	7	22.32±1.40	j
20F8	-	中印自交系	8	24.72±0.72	k
20F9	-	中印自交系	9	22.22±1.23	j
20F10	贝栗4号	印度南瓜	10	31.40±0.35	l
20F11	香南瓜	印度南瓜	11	19.41±0.90	i
20F22	爱碧斯	印度南瓜	12	19.57±0.43	i
20F23	绿亨银栗	印度南瓜	13	3.23±0.06	a
20F24	新红板栗	印度南瓜	14	11.24±0.10	f
PG1	P70	印度南瓜	15	14.79±0.50	h
20F25	香芋南瓜	中国南瓜	16	20.97±1.35	j
PG10	-	中国南瓜	17	4.16±0.34	ab
Wyr	-	中国南瓜	18	3.42±0.11	a
20F16	N2*20F3	自配组合	19	13.05±0.27	g
20F17	MO*20F5	自配组合	20	7.32±0.10	e
20F18	甬南甜4号	自配组合	21	11.91±1.77	fg
20F19	N4*20F7	自配组合	22	21.08±0.06	j
20F20	甬南甜3号	自配组合	23	12.95±0.30	g

代号	商品名	类型	编号	类胡萝卜素
20F1	-	中印自交系	1	5.12±0.34	bc
20F2	-	中印自交系	2	7.04±0.46	de
20F3	-	中印自交系	3	5.62±0.19	cd
20F4	-	中印自交系	4	4.93±0.17	bc
20F5	-	中印自交系	5	6.70±0.78	de
20F6	-	中印自交系	6	6.44±0.28	cde
20F7	-	中印自交系	7	22.32±1.40	j
20F8	-	中印自交系	8	24.72±0.72	k
20F9	-	中印自交系	9	22.22±1.23	j
20F10	贝栗4号	印度南瓜	10	31.40±0.35	l
20F11	香南瓜	印度南瓜	11	19.41±0.90	i
20F22	爱碧斯	印度南瓜	12	19.57±0.43	i
20F23	绿亨银栗	印度南瓜	13	3.23±0.06	a
20F24	新红板栗	印度南瓜	14	11.24±0.10	f
PG1	P70	印度南瓜	15	14.79±0.50	h
20F25	香芋南瓜	中国南瓜	16	20.97±1.35	j
PG10	-	中国南瓜	17	4.16±0.34	ab
Wyr	-	中国南瓜	18	3.42±0.11	a
20F16	N2*20F3	自配组合	19	13.05±0.27	g
20F17	MO*20F5	自配组合	20	7.32±0.10	e
20F18	甬南甜4号	自配组合	21	11.91±1.77	fg
20F19	N4*20F7	自配组合	22	21.08±0.06	j
20F20	甬南甜3号	自配组合	23	12.95±0.30	g

提取液	浸提时间/min	类胡萝卜素含量	最高点提取2次的含量
乙醇	0	5.01±0.40	-
	30	6.51±0.32	-
	60	6.74±0.34	-
	90	8.04±0.07	8.74±0.12
	120	7.14±0.34	-
	150	7.01±0.34	-
	180	6.67±0.38	-
丙酮	0	5.40±0.18	-
	30	6.07±0.10	-
	60	6.93±0.26	8.19±0.06
	90	6.84±0.12	-
	120	6.53±0.06	-
	150	6.09±0.16	-
	180	5.87±0.21	-

提取液	浸提时间/min	类胡萝卜素含量	最高点提取2次的含量
乙醇	0	5.01±0.40	-
	30	6.51±0.32	-
	60	6.74±0.34	-
	90	8.04±0.07	8.74±0.12
	120	7.14±0.34	-
	150	7.01±0.34	-
	180	6.67±0.38	-
丙酮	0	5.40±0.18	-
	30	6.07±0.10	-
	60	6.93±0.26	8.19±0.06
	90	6.84±0.12	-
	120	6.53±0.06	-
	150	6.09±0.16	-
	180	5.87±0.21	-

浸提温度/℃	类胡萝卜素含量 /(mg/100 g)	料液比 /(g/mL)	类胡萝卜素含量 /(mg/100 g)
30	6.35±0.12	1:8	5.52±0.04
40	6.35±0.00	1:16	6.36±0.10
50	6.45±0.05	1:24	6.37±0.35
60	7.06±0.10	1:32	7.73±0.01
70	6.82±0.09	1:40	7.37±0.09