Abstract:

In order to better understand the effect of rooting medium on the rooting and growth of medicinal Dendrobium in tissue culture and rapid propagation, find a method for rapid rooting culture of medicinal Dendrobium, and cultivate high-quality and strong seedlings of medicinal Dendrobium, three rooting media were designed to add 85 g / L banana mud, 85 g / L potato mud and no nutrients, with D. officinale and D. huoshanense as materials. The results showed that the addition of nutrients was necessary for the rooting and growth of medicinal Dendrobium tissue culture seedlings, and played a good promoting role in the rooting and growth of tissue culture seedlings. The addition of 85 g/L bananas was the best for rooting of D. officinale tissue cultured seedlings. The addition of 85 g/L potatoes was most beneficial for the rooting of tissue cultured D. huoshanense seedlings. Suitable rooting medium can achieve high efficiency when cultivating seedlings of different types of medicinal Dendrobium.

Key words: medicinal Dendrobium, Dendrobium officinale, Dendrobium huoshanense, tissue culture, nutrient, rooting medium, growth performance